血必净口服泡腾片对内毒素致家兔发热及弥漫性血管内凝血模型的影响

Effect of Xuebijing oral effervescent tablet on endotoxin induced fever and disseminated intravascular coagulation rabbit model

观察血必净口服泡腾片对内毒素致家兔高热和弥漫性血管内凝血(DIC)的影响,为揭示其治疗感染性疾病的机制提供实验依据。本研究采用激光散斑血流视频监测系统监测家兔耳廓微循环血流,采用血凝仪检测凝血功能指标,酶联免疫法检测纤溶系统功能,HE染色病理切片观察微血栓形成。在内毒素致家兔发热模型上,血必净口服泡腾片4.8、2.4及1.2 g.kg 1剂量组在给药后1 h即有明显的解热作用,作用可持续到给药后3 h,与模型对照组比较具有显著差异(P<0.05)。模型对照组家兔耳廓血流灌注量(54.45±14.53)PU明显低于正常对照组(77.18±12.32)PU,两组比较具有显著差异(P<0.05);血必净口服泡腾片4.8、2.4及1.2 g.kg 1剂量组均可增加血流灌注量,其中1.2 g.kg 1剂量组与模型对照组比较具有显著差异(P<0.05);模型对照组家兔血浆中PAI1含量(30.48±2.46)ng.mL 1明显高于正常对照组(20.93±3.25)ng.mL 1,模型对照组FIB含量(3.34±1.09)g.L 1明显低于正常对照组(4.84±1.10)g.L 1,两组比较具有显著差异(P<0.05,P<0.01),4.8及2.4 g.kg 1剂量组可降低家兔血浆中PAI1含量、升高FIB含量,与模型对照组比较具有显著差异(P<0.05,P<0.01)。结果提示,血必净口服泡腾片具有治疗感染性高热和DIC的药理作用,其机制与改善感染后微循环障碍、抑制纤溶系统激活,从而抑制凝血、减少微血栓形成有关。

In order to discover the mechanism of Xuebijing oral effervescent tablet （XBJOET） to treat infectious diseases, the effect of XBJOET on endotoxin induced rabbit fever and disseminated intravascular coagulation （DIC） was investigated. Auricle microcirculation in rabbit was detected by laser speckle blood perfusion imager system; coagulation function was measured by coagulation analyzer, fibrinolytic system was quantified by Elisa assay and micro thrombosis in tissues was observed with HE staining under light microscope. The results demonstrated that the body temperature of rabbit decreased significantly at 1-3 h after administration with 4.8, 2.4 and 1.2 g.kg^-1 XBJOET to endotoxin induced DIC rabbit model, the auricle microcirculation blood flow in model group （54.45±14.53） PU was lower than that in control group （77.18 ±12.32） PU. The auricle microcirculation blood flow increased markedly and there was significant difference between model group and 1.2 g.kg^-1 XBJOET group. There was significant difference between model group and control group in the content of PAIl and FIB. The PAIl levels in model and control groups were （30.48 ±2.46） ng.mL^-1 and （20.93±3.25） ng.mL^-1, respectively. The FIB levels in model and control group were （3.34 ± 1.09） g.L^-1 and （4.84±1.10） g.L^-1, respectively. The content of PAIl in rabbit plasma decreased notably, there were significant differences between model group and 4.8, 2.4 g .kg^-1 XBJOET groups. On the contrary the content of FIB increased. XBJOET possessed pharmacological activities of curing infectious fever and DIC, the mechanism of which is related to amelioration of microcirculation disturbance, inhibition of fibrinolytic system activation and coagulation and micro thrombosis elimination.