太原地区鸡源性大肠杆菌的分离鉴定及耐药性分析

Isolation and identification of antibiotic resistance Escherechia coli from chicken farms in Taiyuan areas

为调查太原市周边地区鸡源大肠杆菌(E.coli)的流行情况,本研究从规模化养鸡场采集患腹泻病鸡的粪便样品进行E.coli分离、血清型鉴定及耐药性检测,通过接合试验检测质粒在抗生素抗性水平转移中的作用。结果表明:分离到81株E.coli属于15种不同血清型,其中28株属于5种优势血清型,即O1、O8、O78、O84和O143;所有分离菌株对13种抗菌药物均有不同程度的耐药性,其中qnrA、tetM、tetA、sul2、strA、aadA、floR为检测到的主要耐药基因;分离菌株质粒携带的7种耐药基因可通过接合作用转移。该研究结果证明E.coli对常用抗生素的耐药已普遍存在,其携带的耐药基因可通过接合作用在细菌之间传递。

To investigate the serotype, drug resistance and determine the transfer resistance in Escherechia coli around Taiyuan area, a total of 81 E. coli were isolated fi＇om the fecal samples collected in different chicken farms. The results showed that these E.coli isolates belonged to 15 kinds of serotypes, of which 28 isolates distributed in 5 O serotypes, i.e. O1, 08, 078, 084 and O143. In addition, all the isolates had different degrees of resistance to 13 kinds of antibacterial drugs tested in this study, PCR detections displayed that 7 drug resistant gene including qnrA, tetM, tetA, sul2, strA, aadA and floR were mainly found in these isolates. Furthermore, the 7 drug resistance genes were able to transfer into the drug sensitive E.coli NK5449 strain by bacterial conjugations which indicated that drug resistances were transferred by plasmid borne drug resistant genes.