2011年河南省猪流行性腹泻病毒流行株的遗传进化分析

Phylogenetic analysis of porcine epidemic diarrhea virus isolated from Henan province in 2011

2011年前后,猪流行性腹泻(PED)在河南省爆发流行。为探明该病再次流行的原因,本研究对2011年收集的7份来自河南省不同地区PED病毒(PEDV)流行病毒株M基因进行RT-PCR扩增、克隆、测序及分析。序列分析显示,7株PEDV河南流行株M基因部分核苷酸及氨基酸发生变异,不同于以往参考病毒株。基于M基因的遗传进化分析显示,PEDV河南流行株和参考株可以分为3个群(G1、G2、G3),7株PEDV河南流行株均属于第3群,与2010年中国BJ2010株、2007年韩国PFF188株及2008年泰国08RB03株亲缘关系密切,而与欧洲病毒株(CV777、Br1/87)、1995年和2004年泰国株(M_NIAH1795_04、M_NIAH2013_95)及2006年中国LZC株亲缘关系较远。结果表明,2011年在中国河南省及其它地区流行的PEDV是一个新的基因型,可能起源于韩国和泰国。

Porcine epidemic diarrhea virus （PEDV） has been reemerging in swine herds of Henan province since late 2010. The M gene of seven PEDVs isolated from swine herds in Henan province in 2011 were amplified, cloned, sequenced and compared with each other as well as with reference PEDV strains. Sequence analysis revealed that the PEDV Henan isolates had several nucleotides and amino acids mutated, which were different fxom reference strains. Phylogenetic analyses showed that all Henan field isolates had close relationships with Korea PFF188 strain （2007）, Thailand 08RB03 strain （2008） and Chinese B J2010 strain （2010）, but differ genetically from European strains （Brl/87,CV777）, Thailand strains （M_NIAH1795 04, M_NIAH2013_95） isolated in 1995 and 2004 and China strain （LZC） isolated in 2006. The results show that the PEDVs isolated in Henan province in 2011 are new variants and might origin from Korea and Thailand strains.