转化生长因子β调节培养的人角膜基质细胞MMP-2和MMP-9的分泌

TGF-β modulates secretion of MMP-2 and MMP-9 by cultured human corneal keratocytes

目的观察转化生长因子６（ＴＧＦ－β）对培养的人角膜基质细胞分泌ＭＭＰ－２和ＭＭＰ－９的调节作用，探讨ＴＧＦ－β治疗非感染性角膜溃疡的潜在可能性。方法采用酶谱法检测人角膜基质细胞ＭＭＰ－２和ＭＭＰ－９的分泌，并观察 ＴＧＴ－β对 ＭＭＰ－２和 ＭＭＰ－９分泌的调节作用。结果培养的人角膜基质细胞分泌 ＭＭＰ－２和 ＭＭＰ－９，ＴＧＦ－β抑制ＭＭＰ－９的活性，且随ＴＧＦ－β质量浓度增加而增强，随ＴＧＦ－β作用时间延长而增强；对ＭＭＰ－２的活性，ＴＧＦ－β有较弱的促进作用。结论ＴＧＦ－β作为一种ＭＭＰ－９的抑制剂，在促进非感染性角膜溃疡愈合方面很有潜力。

Objective To observe modulation of TGF-β for secretion of MMP-2 and MMP-9 by cultured human corneal keratocytes and investigate the possibility of treatment of non-infectious corneal ulcer with TGF-β. Methods The second～ third passage cells were seeded at proper density, incubated with TGF-βof different concentration (0, 0. 1 1, 5 ng/ml) or for different time (24, 48, 72h). The supernatants were collected and checked. Zymography was applied to test secretion of MMP-2 and MMP-9 by human corneal keratocytes. To observe the TGF-β's effect on the secretion, the zymography results were examined by densitometric analysis. Results Cultured human corneal keratocytes secreted MMP-2 and MMP-9. TGF-β inhibited the activation of MMP-9 and the inhibition was dose-dependent and time-dependent. TGF-β mildly promoted the activation of MMP-2. Conclusion Cultured human corneal keratocytes secrete MMP-2 and MMP-9. Being an inhibitor of MMP-9,TGF-β may be very potential in improving the healing of non-infectious corneal ulcer.