牛磺酸对糖尿病性白内障防治的实验研究

The experimental study of taurine on diabetic Cataract

目的探讨牛磺酸对链脲佐菌素（ＳＴＺ）诱导的大鼠糖尿病性白内障的防治效果。方法１４０只ＳＤ大鼠随机分为６组，实验组通过口服、点眼、结膜下注射等途径补充牛磺酸，定期观察晶状体的变化，测定血糖及晶状体中山梨醇、超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ）含量并与对照组相比较。结果白内障时晶状体中山梨醇及ＭＤＡ含量显著增加，ＳＯＤ活力明显下降。各牛磺酸用药组晶状体中山梨醇含量无显著性改变，但ＳＯＤ活力明显增加，ＭＤＡ含量明显降低，白内障发生时间推迟，混浊程度减轻，全身应用牛磺酸还可以降低血糖。结论牛磺酸对ＳＴＺ诱导的糖尿病性白内障有一定的防治作用。

Objective To evaluate the effects of taurine on streptozotocin (STZ ) -induced diabetic cataract. Methods 140 SD rats were randomly divided into 6 groups: normal control, cataract, preventive, oral treatment, eye drop application and subconjunctival injection groups. Diabetic cataract was induced by STZ intraperitoneal injection (70 mg/kg in 0.1mol/L sodium citrate buffer). Right after STZ injection, prevention group received 1% taurine solution in drinking water. After two weeks of STZ injection,the last three groups were given 1% taurine solution drinking,6. 5% taurine drops in conjunctival sac (four times a day) and 6. 5% taurine as subconjunctival injection (two times a week), respectively. Lenses were examined using a slit lamp once a week. At the end of 2 weeks,4 weeks,8 weeks after STZ injection,blood was collected to measure the blood glucose, and lenses were isolated to measure the content of sorbitol, superoxide dismutase (SOD) and malondialdehyde (MDA). Results in cataractous lenses the content of sorbitol and MDA were markedly increased. In each group of using taurine,there were no significant changes in the levels of sorbitol,while the activities of SOD improved,the quantity of MDA decreased and the lens opacity was delayed and the opacity degree was obviously slight. Meanwhile,blood glucose level could be lowered through oral taurine. Conclusion Taurine can lower blood glucose,inhibit lens lipid peroxide reaction,stabilize the structure of cells and regulate the osmotic pressure within the lens, so it can prevent or delay cataract formation and development in STZ-induced diabetic rats.