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抑制ATR基因可提高HeLa细胞对烷化剂的敏感性 被引量：1

Expression Inhibition of ATR Gene Improve the Susceptibility of HeLa Cells to Alkylating Agents
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摘要 目的:探讨ATR基因的表达对HeLa细胞对烷化剂敏感性的影响。方法:采用RNA干扰技术抑制HeLa细胞ATR基因的表达,观察ATR被阻断后HeLa细胞对烷化剂敏感性的变化,从而确定ATR基因的作用。结果:筛选到表达针对ATR基因的短发夹RNA(shRNA)阳性克隆,Western印迹结果显示ATR基因表达受到明显抑制;HeLa细胞对烷化剂的敏感性实验提示,ATRshRNA+HeLa细胞对烷化剂的敏感性明显增强。结论:抑制ATR基因可明显提高HeLa细胞对烷化剂的敏感性。 Objective： To investigate the effect of the expression of ATR gene on HeLa cells＇ susceptibility to al-kylating agents. Methods： RNA interference technology was employed to suppress the expression of ATR, then the role of ATR in HeLa cells＇ anti-alkylation ability was inspected. Results： A positive clone of siRNA against ATR gene was successfully screened and it can strikingly inhibit the expression of ATR in HeLa cells by Western blot analysis, and then the ability of HeLa cells to resist alkylating agents was significantly attenuated. Conclusion： The inhibition of the expression of ATR gene can significantly improve the HeLa cells＇ susceptibility to alkylating agents.
作者 王颖丽 让文亮 张雪 檀英霞 李素波 鲍国强 高红伟 宫锋 季守平
机构地区 军事医学科学院野战输血研究所
出处 《生物技术通讯》 CAS 2013年第4期501-503,535,共4页 Letters in Biotechnology
基金 国家自然科学基金(30671995)
关键词 ATR基因 烷化剂 HELA细胞 ATM-Rad3-related gene alkylating agent HeLa cells
分类号 Q78 [生物学—分子生物学]
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参考文献8

  • 1Ksteller M, Herman J G. Generating mutation but providingchemosensitivity: the role of ()6-methylguanine-l)NA methyl-tmnsferase in human cancer[J]. Oncogen, 2004.23:1-8.
  • 2章扬培.甲基转移酶与肿瘤耐药预见性、个体化化疗的研究[J].癌症,2004,23(6):724-734. 被引量：37
  • 3Brell M, Torlosa A, Verger K, et al. Prognostic1 significanceof (/-raethylguanine-DNA methyllransferase deterniined bypromoter hypermethylation and immunohistocheniical expres-sion in anaplastic gliomas[J]. Clin Cancer Kes, 2005,11(14):5167-5174.
  • 4Sperka T, Wang J, Rudolph K L. DNA damage checkpointsin stem cells, ageing and cancer[J], Nat Rev Mol Cell Biol,2012,13(9):579-590.
  • 5Berdelle N, Nikolova T, Quiros S, et al. Artesunate inducesoxidative DNA damage, sustained DNA double-strand breaks,and the ATM/ATR damage response in cancer cells[J]. MolCancer Ther, 2011,10(12):2224-2233.
  • 6Collis S J, Swartz M J,Nelson W G, et al. Enhanced radia-tion and chemotherapy-mediated cell killing of human cancercells by small inhibitory RNA silencing of DNA repair factors[J]. Cancer Res, 2003,63:1550-1554.
  • 7Shiotani B, Zou L. A human cell extract-based assay for theactivation of ATM and ATR checkpoint kinases[J]. MethodsMol Biol, 2011,782:181-191.
  • 8Collis S J, Boulton S J. Emerging links between the biologi-cal clock and the DNA damage response[J]. Chromosoma,2007,116(4):331-339.

二级参考文献28

  • 1Pegg AE. Methylation of the O6 position of guanine in DNA is the most likely initiating event in carcinogenesis by methylating agents [J] . Cancer Investigation, 1984, 2:223 - 231.
  • 2McCarthy TV, Karran P, Lindahl T.Inducible repair of O9-alkylated DNA pyrimidines in Escherichia coli [J]. EMBO J, 1984, 3:545 - 550.
  • 3Teo I, Sedgwick B, Demple B, et al.Induction of resistance to alkylating agents in E. coli: the ada + gene product serves both as a regulatory protein and as an enzyme for repair of mutagenic damage [J]. EMBOJ, 1984,3(9):2151-2157.
  • 4Scudiero DA, Meyer SA, Clatterbuck BE,et al. Sensitivity of human cell strains having different ability to repair O6-methylguanine in DNA to inactivation by alkylating agents including chloroethylnitrosourea [J]. Cancer Res, 1984, 44:2467 - 2474.
  • 5Tsujimura T, Zhang Y, Fujio C, et al.O6-methylguanine-DNA methyltransferase activity and sensitivity of Japanese Tumor Cell Strains to ACNU [J]. Japan J Cancer Res, 1987, 78:1207 - 1215.
  • 6Watatani M, Ikenaga M, Hatanaka T, et al. Analysis of MNNG-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer- xenografts in athymic nude mice [J]. Carcinogenesis,1985, 6:549 - 553.
  • 7Mattern J, Eichhorn U, Kaina B, et al.O6-methylguanine-DN A methyltransferase activity and sensitivity to cyclophosphamide and cisplatin in human lung tumor xenografts [J]. Int J Cancer, 1998, 77:919 -922.
  • 8Piccioni D, D'Atri S, Papa G, et al.Cisplatin increases sensitivity of human leukemic blasts to triazene compounds [ J].J Chemother, 1995, 7(3): 224-229.
  • 9Preuss I, Thust R, Kaina B. Protective effect of O6-methylguanine-DNA methyltransferase (MGMT) on the cytotoxic and recombinogenic activity of different antineoplastic drugs [J] .Int J Cancer,1996, 65(4): 506 -512.
  • 10Chen SS, Citron M, Spiegel G, et al.O6-methylguanine-DNA methyltransferase in ovarian malignancy and its correlation with postoperative response to chemotherapy [J]. Genecol Oncol, 1994, 52(2): 172 - 174.

共引文献36

同被引文献14

  • 1魏嘉,刘宝瑞,王亚平,钱晓萍.DNA修复基因单核苷酸多态性与铂类药物抵抗研究进展[J].中华肿瘤杂志,2006,28(3):161-163. 被引量：31
  • 2Tewari KS, Monk BJ. American Society of Clinical Oncology 2011 Annual Meeting update: summary of selected gynecologic cancer abstracts [ J ]. Gyneeol Oncol, 2011, 122 (2) :209-212.
  • 3Heintz AP, Odieino F, Maisonneuve P, et al. Carcinoma of the ovary: FIGO 26th Annual Report on the Results of Treatment in Gynecological Cancer [ J ]. Int J Gynaecol Obstet, 2006, 95 ( Suppl 1 ) :S161-S192.
  • 4Bookman MA, Brady MF, MeGuire WP, et al. Evaluation of new platinum-based treatment regimens in advanced-stage ovarian cancer: a Phase 11I Trial of the Gynecologic Cancer Intergroup [J]. J Clin Oncol, 2009, 27(9) :1419-1425.
  • 5Hartmann JT, Lipp HP. Toxicity of platinum compounds [ J ]. Expert Opin Pharmaeother, 2003, 4(6) :889-901.
  • 6Saleh EM, E1-Awady RA, Anis N, et al. Induction and repair of DNA double-strand breaks using constant-field gel electrophoresis and apoptosis as predictive markers for sensitivity of cancer cells to cisplatin[J]. Biomed Pharmacother, 2012, 66(7) :554-562.
  • 7Sears CR, Turchi JJ. Complex cisplatin-double strand break (DSB) lesions direcdy impair cellular non-homologous end-joining (NHEJ) independent of downstream damage response ( DDR )pathways[J]. J Biol Chem, 2012, 287(29) :24263-24272.
  • 8Huehls AM, Wagner JM, Huntoon CJ, et al. Identification of DNA repair pathways that affect the survival of ovarian cancer cells treated with a poIy (ADP-ribose) polymerase inhibitor in a novel drug combination [ J ]. Mol Pharmaeol, 2012, 82 (4) :767-776.
  • 9Cimprieh KA, Cortez D. ATR: an essential regulator of genome integrity[ J]. Nat Rev Mol Cell Biol, 2008, 9 (8) :616-627.
  • 10Nam EA, Cortez D. ATR signalling: more than meeting at the fork [J]. Bioehem J, 2011,436(3) :527-536.

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生物技术通讯
2013年 第4期

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