摘要
目的:利用大鼠评价酶解转变的人红细胞的安全性。方法:人红细胞与大鼠血清进行常规配血,检测二者之间的相容性;将人B型红细胞酶解后输注大鼠,流式细胞术检测人红细胞在大鼠体内的存活率。结果:人红细胞与大鼠血清不凝集,但人红细胞输注大鼠体内很快被排斥,1 h下降至50%以下,18 h被完全排斥。结论:仅依靠红细胞配血实验来选择人红细胞输血动物模型是不全面的,大鼠不适合作为酶解转变的人红细胞安全性评价的动物模型。
Objective： To study the safety of enzymatically converted group O red blood cells（ECORBC） by rat model. Methods： The compatibility between human red blood cells（RBC） and rat was detected by blood match- ing. The B group RBC after enzymatic conversion were transfused to rats, and the survival rate of the ECORBC af-ter transfusion was detected. Results： There was no agglutination after human RBC mixed with rat blood serum. ECORBC were rejected by rats within short time, the survival rate of ECORBC decreased below 50% at 1 hour and about zero at 18 hours. Conclusion： Blood matching is not enough to select the animal model for human RBC transfusion, the rat is not suitable to appreciate the ECORBC safety.
出处
《生物技术通讯》
CAS
2013年第4期543-545,共3页
Letters in Biotechnology
关键词
血型转变
通用型红细胞
输血
动物模型
blood group conversion
universal red blood cells
transfusion
animal model
