重组γ-干扰素作用人Tenons囊成纤维细胞体外研究

Study of recombinant human IFN-γ effect on cultured human fibroblasts from Tenons capsule

目的 体外研究人重组γ－干扰素对人Ｔｅｎｏｎｓ囊成纤维细胞作用。方法 以人Ｔｅｎｏｎｓ囊成纤维细胞作为实验对象，分别加入０．１～１０＾６ Ｕ／ｍｌ人重组γ－干扰素（ＩＦＮ－γ）、０．００１～１０ｍｇ／Ｌ丝裂霉素（ＭＭＣ）、０．１～１０３ｍｇ／Ｌ５－氟尿嘧啶（５－Ｆｕ）孵育４８ｈ，然后以ＭＴＴ法进行检测。结果 高浓度和低浓度ＩＦＮ－γ实验组ＯＤ值较对照组升高（Ｐ〈０．０５）。

Objective To study the effect of recombinant human interferon-γ (IFN-γ ) on in vitro cultured human fibroblasts from Tenons capsule. Methods The effect of 0.1-10U/ml human IFN-γ and 0.001-10 mg/L mitomycin-C (MMC),0.1-10mg/L 5-fluorouracil(5-Fu) on cultured human Tenons capsule fibroblasts (HTCF) was studied by using a MTT [3-(4,5-dimethylthiazo-2-yi) -2,5-diphenyltetrazolium bromide; Thiazolyl blue] colorimetric assay. The incubation time of these drugs was 48 h. The effect of IFN-γ on the fibroblasts was repeated in the second experiment using the same method. The results were analyzed using one-way analysis of variance (ANOVA) of the statistical package for social sciences (SPSS ) 9.0 version,the difference was considered to be significant if P<0.05. Results By analyzing OD values of first experiment, one- way ANOVA showed: (i) significant difference between 10 U/ml of IFN and control(P=0. 02 ),1 U/ml of IFN and control (P = 0. 006 ) (OD values increased in the experimental group), but no significant difference between other concentration of IFN and control (P>0. 05) (OD values increased or decreased in the experimental group); (11 ) significant difference between 10mg/L, 1mg/L,0. 1 mg/L MMC and control (P=0.000), between 0. 01 mg/L MMC and control (P=0. 033), while no significant difference between 0.001 mg/L MMC and control (P=0. 413); (iii) significant difference between 1000 mg/L, 100 mg/L 5-Fu and control (P=0. 000 ),while no significant difference between other concentrations of 5-Fu and control (P>0. 05). As to the results of second experiment, one-way ANOVA showed significant difference between 10/ml,0.1 U/ml of IFN and control (P< 0. 005 ) (OD values of IFN increased in the experimental group), 10U/ml, 10 U/ml of IFN and control (P<0.05) (OD values of IFN decreased in the experimental group),but no significant difference between other concentrations of IFN and control (P> 0. 05 ). The inhibition rate of IFN was smaller than that of MMC and 5-Fu(P<0.005). Conclusion IFN-γ has bifunctional effect (both enhancement and inhibition) on proliferation of cultured HTCF. The antiproliferative effect on HTCF of IFN-γ is weaker than MMC and 5-Fu.