用原位杂交法检测单纯疱疹病毒Ⅰ型在潜伏感染时的少量潜伏相关转录本表达

Detection of herpes simplex virus type Ⅰ minor latency-associated transcript during latent infection in trigminal ganglia by in situ hybridization

目的探讨单纯疱疹病毒Ⅰ型在三叉神经节内潜伏感染时的基因表达。方法以 ＢＡＬＢ／ｃ小鼠制备单纯疮疹性角膜炎，种毒后３６天取三叉神经节和角膜，用免疫组化法检测病毒的抗原表达。制备ｃＲＮＡ探针，用原位杂交法检测 ｍ－ＬＡＴ的表达。结果种毒后３６天，免疫组化结果为阴性，原位杂交结果为阳性。结论 ＨＳＶ－１在三叉神经节内潜伏感染时基因的转录并未静止，有ｍ－ＬＡＴ基因的表达。

Objective To detect the HSV-1 RNA expression in latent infection in trigminal ganglia. Methods HSV-1 KOS strain was inoculated on BALB/c mice to make the model of murine herpes simplex keratitis(HSK). 36 d after inoculation, trigeminal ganglia and cornea were removed to detect the HSV-1 gC antigen expression by immuno-histochemistry. The digoxigenin-labelled RNA probe was used,m-LAT was detected by in situ RNA hybridization. Results 36 d after inoculation,no positive signals was detected by immuno-histochemisty, and positive signals with antisence probe were detected by in situ hybridization. Conclusion HSV-1 RNA expression was not completely quiet during latent infection, and the m-LAT was positive.