不同血清微环境培养的施万细胞增殖状况比较

Comparison of Schwann Cell′s Proliferation in Different Serum Microenvironment Mediums

目的将不同浓度大鼠血清、10%胎牛血清培养以及无血清培养的大鼠原代施万细胞的增殖状况进行比较,探讨适合施万细胞生长的血清微环境。方法将所培养的原代施万细胞分成5%大鼠血清组、10%大鼠血清组、15%大鼠血清组、20%大鼠血清组、10%胎牛血清组、无血清组,另设不含细胞的空白对照组。采用CCK-8细胞增殖试剂,分别于培养后24、48、72、96、120h对各组的细胞增殖情况进行测定。结果 5%大鼠血清组的细胞在96h内的生长状况与10%胎牛血清组相比无显着差异。结论在96h内,5%的大鼠血清浓度最适合该细胞生长。

Objective To comparie the generation of rat Schwann cells＇s proliferation in different Serum microenvironment medi- ums, include different concentrations of rat serum, 10% fetal bovine serum and serum free, to explore suitable for schwann cells growth ofserum microenvironment. Methods The original generation Schwann cells were grouped into 5% rat serum group, 10% rat serum group, 15% rat serum group, 20% rat serum group, 10% fetal bovine serum group, the serum free group, the blank control group. CCK - 8 cell proliferation reagent was wsed to test each cell proliferation condition in the 24, 48, 72, 96,120h. Results The growth status of ceils during 96h, in 5% rat serum group had no significant differences compared with 10% fetal bovine serum group. Conclusion During 96h, 5% of rat serum concentration is most suitable for the cell growth.