浙东地区汉族人群sLOX-1浓度与LOX-1基因多态性相关性研究

Correlation Analysis of Plasma Soluble LOX-1 Levels and Gene Polymorphism of Lectin-like Oxidized Low-density Lipoprotein Receptor 1(LOX-1)

目的观察血浆中sLOX-1浓度是否和LOX-1基因多态性相关。方法采用酶联免疫吸附法测定354例接受冠状动脉造影的浙东地区汉族人群住院患者血清中sLOX-1的浓度,并采用Tm-shift基因分型方法对G501C和3’UTR-C/T等位基因型进行检测,其中包括43例急性心肌梗死、86例不稳定型心绞痛、89例稳定型心绞痛和136例经冠状动脉造影排除冠心病的患者。结果 3'UTR-C/T位点TT,TC,CC基因型分别为5.4%,37.3%,57.3%,501G/C位点CC,GC,GG基因型分别为5.4%,31.9%,62.7%,3'UTR-C/T TT,TC,CC 3组sLOX-1浓度分别为160.0±56.1,132.7±52.0,128.9±58.0pg/ml,501G/C CC,GC,GG 3组sLOX-1浓度分别为134.0±55.2,127.2±50.8,134.2±58.7pg/ml。不同基因型患者sLOX-1浓度各组间没有显著性差异,在各亚组间也没有差异。结论浙东地区汉族人群sLOX-1浓度和LOX-1基因多态性无相关性。

Objective To assess whether the level of serum soluble lectin - like oxidized low - density lipoprotein receptor - 1 （ sLox - 1 ） is associated with LOX - 1 gene polymorphism. Methods Levels of sLox - 1 were measured in 345 patients of Han population of eastern area of Zhejiang Province who come to our centre seeking for coronary angiography with enzyme - linked immunosorbent assay, including 45 acute myocardial infarction patients, 86 unstable angina pectoris patients,89 stable angina pectoris patients and 136 non- coronary patients excluded by coronary arteriography. The polymorphisms of gene LOX - 1 G501C and 3＇UTR - C/T were measured by the way High - throughput SNP genotyping with single - tube PCR with Tm - shift primers. Results The frequencies for the 3＇UTR - C/T TT, TC, CC were 5.4% , 37.3% , 57.3% , and G501C CC, GC, GG were 5.4% , 31.9% , 62.7% , respectively. The level of sLox- 1 were 160.0 ±56.1, 132.7 ±52.0, 128.9 ± 58.0pg/ml in 3＇UTR -C/T TT, TC, CC group, and 134.0 ±55.2, 127.2 ±50.8, 134.2 ± 58.7pg/ml in 501G/C CC, GC, GG group. There were no discrepancy in plasma sLOX - 1 levels between different groups. In sub- group analysis, there were no discrepancy too. Conclusion Levels of sLox - 1 were not associated with the polymorphisms of gene LOX - 1 in Han population of eastern area of Zhejiang Province.