苦参碱对视网膜母细胞瘤细胞survivin和端粒酶活性表达的影响

Effect of Matrine on Telomerase Activity and Expression of Survivin in Retinoblastoma

目的研究苦参碱对视网膜母细胞瘤(RB)细胞凋亡抑制蛋白survivin和端粒酶活性表达的影响,探讨苦参碱对Rb的作用机制。方法体外培养视网膜母细胞瘤患者HXO-Rb细胞经不同浓度的苦参碱作用后,采用噻唑蓝比色法测定细胞生长情况,RT-PCR检测survivin RNA水平,聚合酶链法(PCR)检测端粒酶活性。结果苦参碱在一定范围内可抑制HXO-Rb细胞的生长,使survivin mRNA表达下调。可明显观察到细胞凋亡的形态学改变,端粒酶活性明显下降,细胞凋亡率增加。结论苦参碱可以增加HXO-RB细胞凋亡,可以减少RB细胞mRNA表达,减弱RB细胞端粒酶活性,影响其周期变化,抑制RB细胞系增殖。其对survivin的抑制,及端粒酶活性的下降可能是诱发RB细胞凋亡的原因。

Objective To study the effect of matrine on telomerase activity and the expression of survivin, an inhibitor of apoptosis, in retinoblastoma （Rb） , so as to explore the action mechanism of matrine on Rb. Methods HXO - Rb cells of Rb patients were cultured in vitro, followed by treatment with matrine at different concentrations. MTT eolorimetry method, RT - PCR and PCR were used to meas- ure the growth condition of ceils, the level of survivin RNA and the telomerase activity, respectively. Results Matrine could inhibit the growth of HXO - Rb cells within limits to down - regulate the expression of survivin mRNA. Morphological changes of apoptosis were observed clearly. The telomerase activity was significantly decreased and the apoptosis rate was increased. Conclusion Matrine can increase the apoptosis of HXO -Rb cells, reduce the expression of mRNA in Rb cells, weaken the telomerase activity and influence the cell cycle, thus inhibiting the proliferation of Rb cell line. Reasons for Rb apoptosis may be the inhibition on survivin and the decrease of telomerase activity.