吗啡诱导条件位置性偏爱大鼠海马CA1区NR2A、NR2B的变化

Changes in Protein Expression of NR2A and NR2B Subunit in Hippocampal CA1 of Morphine-induced CPP Rats

目的检测吗啡诱发条件位置性偏爱(conditioned place preference,CPP)大鼠海马CA1区NMDA受体亚型NR2A、NR2B的变化,探讨NR2A、NR2B在吗啡精神依赖形成过程的作用与可能机制.方法采用恒量法(10mg/kg)连续颈背部皮下注射(subcutaneous,SC)吗啡8 d建立大鼠CPP模型.采用免疫组化法测定海马CA1区NR2A、NR2B的表达.结果 SC 10 mg/kg吗啡8 d建立大鼠CPP模型,吗啡组海马CA1区NR2A表达与生理盐水组比较无显著性变化(P>0.05),而NR2B表达较生理盐水对照组增加(P<0.05).结论吗啡诱导大鼠CPP海马CA1区NR2B表达增加,NR2B可能参与吗啡诱导CPP形成.

Objective To detect the changes of NMDA receptor subunit NR2A and NR2B in hippocampal CA1 area of morphine-induced CPP rats and explore the function and possible mechanism of NR2A and NR2B in morphine psychological dependence. IMethods Morphine was administered via subcutaneous injection at constant dose （10 mg/kg） for 8 days to establish morphine CPP. The expression of NR2A and NR2B in hippocampal CA1 was detected by immunohistochemistry. Results CPP model was established successfully after injection of morphine （ 10 mg/kg） for 8 days. Compared with the saline group, the expression of NR2A had no significant difference （P〉0.05） , while the expression of NR2B increased in the morphine group （P〈0.05）. Conclusion The expression of NR2B receptor increased in hippocampal CA1 area of morphine-induced CPP rats, and NR2B may involve in the establishment of CPP model induced by morphine.