内皮素-1与基质金属蛋白酶-2在喉癌中的表达与浸润转移的相关性研究

Expression of ET-1 and MMP-2 in Laryngeal Carcinoma and Their Meanings

目的探讨内皮素-1(ET-1)、基质金属蛋白酶-2(MMP-2)在喉癌中的表达及其与喉癌相关特征的关系.方法应用免疫组化SP法对52例经手术及病理证实的喉癌标本的ET-1、MMP-2表达进行检测,分析ET-1、MMP-2的表达,同时28例声带息肉及19例喉部正常粘膜组织作为对照.结果 ET-1在喉癌中的阳性表达率为71.15%,分别高于对照组中的声带息肉的50.00%和正常粘膜组织中的42.11%(P<0.05);MMP-2在喉癌中的阳性表达率为69.23%,分别高于对照组中的声带息肉的46.43%和正常粘膜组织中的36.84%(P<0.05).喉癌的ET-1、MMP-2表达呈正相关(P<0.05).结论 ET-1、MMP-2的高表达与喉癌的临床病理特征密切相关,可以作为判断喉癌生长的有效指标;在肿瘤侵袭生长过程中,ET-1、MMP-2可能存在协同作用,共同促进喉癌的发生、发展.

Objective To investigate the expressions of endothelin-1 （ET-1） and matrix metallproteinase-2 （MMP-2） in laryngeal carcinoma, and study the correlation of expression and laryngeal carcinoma relevant characteristics. Methods The expressions of ET-1 and MMP-2 in 52 samples of laryngeal carcinoma were detected and analyzed by using the iinmunohistochemical SP method. And 28 samples of vocal polyp and 19 samples of normal laryngeal mucosa tissue were selected as control. Results The intense expression （71.15%） of ET-1 of laryngeal carcinoma patients was significantly higher than that of the control group （P 〈 0.05） And the intense expression （69.23%） of MMP-2 in laryngeal carcinoma patients was significantly higher than that of the control group （P 〈 0.05） There was a positive correlation between the ET-1 and MMP-2 expressions of laryngeal carcinoma. Conclusion The high expressions of ET-1 and MMP-2 were related to the invasion and aggressiveness in laryngeal carcinoma and could be used as effective markers for diagnosis. ET-1 and MMP-2 may be synergies for promoting the formation and development of laryngeal carcinoma.