脑外伤后脑干组织中血管内皮细胞及血浆蛋白分布改变的免疫组化研究

An Immunohistochemical Study on Changes of Vascular Endothelial Cells and Plasma Proteins in Brainstem Following Cerebral Trauma

目的 :探讨脑外伤后脑干组织中血脑屏障和神经细胞的病理学改变。方法 :采用免疫组化染色显示人体脑干组织中的血管内皮细胞 (CD31)和某些血浆蛋白 (白蛋白及IgM )。结果 :1.在正常脑干组织中 ,CD31阳性物呈连续的线条状 ,围成规则的血管腔 ,其周围无明显间隙 ;血浆蛋白位于血管内 ,未见到血浆 /蛋白阳性神经细胞或神经纤维。 2 .脑外伤后 ,脑干中的CD31阳性物呈不规则弯曲的小片段状 ,围成不规则的血管腔 ,其周围间隙明显增宽 ;同时可见到白蛋白及IgM阳性神经细胞和神经纤维。结论 :脑外伤后 ,脑干组织中的血脑屏障受到了破坏 ,而且神经细胞也有不同程度的损伤。

To understand the pathological changes of blood_brain barrier and neural cells in brainstem following cerebral trauma. Methods:The vascular endothelial cells(CD 31 ) and plasma proteins (albumin and IgM) in human brainstem were demonstrated immunohistochemically.Results∶(1) In normal brainstem tissue,the vascular endothelial cells stained with anti_CD 31 antiserum were continuous with each other without obvious perivascular spaces.Positively stained plasma proteins could only be observed within blood vessels,there seemed to be no plasma protein_positive neural cells or nerve fibers.(2) In the brainstem with cerebral trauma,clusters of divorced endothelial cells were observed in blood vessels with perivascular space remarkably widened.Moreover,staining of albumin,IgM positive neural cells and nerve fibers were observed.Conclusion:The blood_brain barrier as well as neural cells in brainstem were damaged after cerebral trauma.