胃癌高、低发区人群中幽门螺杆菌感染儿童抗CagA抗体的比较

A Comparison of Prevalence of Antibody to Cag A in Children with Infection of Helicobacter Pylori between Aeras of High and Low Incidenes

目的 :明确我国胃癌高、低发区普通人群中Hp感染儿童抗CagA抗体的差异。方法 :采用Helicoblot 2 .0系统检测胃癌高发区 (陕两省安塞县 )和低发区 (广东省从化县 )年龄、性别匹配的Hp阳性无症状儿童血清标本各 30例 ,并对两地儿童的胃窦、胃体粘膜组织学进行比较。结果 :(1)安塞县Hp感染儿童血清抗CagA(116kDa)、VacA(89kDa)、35kDa、30kDa、2 6 .5kDa和 19.5kDa抗体阳性率分别为 90 %、30 %、90 %、10 0 %、10 0 %和 56 .6 7% ,从化县分别为 96 .6 7%、30 %、83.33%、86 .6 7%、86 .6 7%和 4 3.33% ,两者比较无显者性差异 (P >0 .0 5)。 (2 )两地儿童胃粘膜慢性炎症、活动性炎症发生率均无统计学差异 (P >0 .0 5) ,但萎缩及淋巴滤泡形成在胃癌高发区有增多的趋势。结论 :我国胃癌高、低发区普通人群从儿童期开始感染的Hp绝人多数为cagApH阳性Hp ,CagA基因不能作为我国胃癌危险性的预测指标。

To make certain whether there is a difference in the seroprevalence of antibody to Cag A in Helicobacter Pylori (H.pylori) infected children in areas with high and low incidences of the infection in China. Methods:Serum specimens from 30 cases of sex and age matched asymptomatic children were sampled out from children population at Ansai County (an area with high incidence of gastric cancer),Shanxi Province,and Conghua County (an area with low incidence of gastric cancer),Guangdong Province,respectively.Serum antibodies to specific antigens of H.pylori were detected using Helico-blot 2.0 Western blot system,the histopathological features of the gastric biopsy samples from the antrum and the body of stomach were assessed by Sydney System. Results:1.At Ansai County the positive rates of serum antibodies to H.pylori specific antigens such as CagA,VacA,antibody of 35kDa,30kDa,26.5kDa and 19.5kDa were 90%,30%,90%,100%,100%and 56.67%,respectively,and that at Conghua County were 96.67%,30%,83.33%,86.67%,86.67% and 43.33%,no difference in the positive rates between the two counties was observed (P>0.05). 2.The incidences of chronic and active gastritis at the two counties were with no statistically significant difference (P>0.05),but there seemed to be a trend of increase in the rates of atrophy and lymphocyte follicle at Ansai county. Conclusion:Our data suggest that the seroprevalence of antibody to CagA is very common in H.pylori-positive children,and CagA gene may not be a predictive marker of gastric cancer in China.