肾移植病人配组淋巴细胞毒抗体的检测

The Detection of Panel Reactive Antibody in Patients to Receive Renal Transplantation

目的 :通过对肾移植病人的配组淋巴细胞毒抗体 (panelreactiveantibodyPRA)的检测 ,了解与分析肾移植病人配组淋巴细胞毒抗体的差异。方法 :以 36名健康成人外周血淋巴细胞作为群体淋巴细胞来源。采用补体依赖微量淋巴细胞毒方法 ,检测病人血清中的配组淋巴细胞毒抗体。结果 :55例病人进行血清淋巴细胞毒交叉配合实验检测 ,只有 1例阳性 1.82 %。而PRA检测 8例病人血清为阳性 (6女 2男 )占 14.5%。同时此群体中 35例病人曾经输血 ,其中PRA阳性有 6例(17.1% ) ,而未经输血病人 2 0例 ,其中有 2例PRA阳性 (10 % )。结论 :男女病人的PRA结果有显著性差异 (P <0 .0 5)。而输血与未输血病人FRA值无显著性差异 (P >0 .0 5)。与淋巴细胞毒交叉配合实验对比较 ,PRA的检测能更准确预测病人体内致敏状况和抗体水平。

To investigate the possible difference in the positive rates of panel reactive antibody (PRA) among different patients to receive renal transplantation. Methods:The peripheral blood lymphocytes from 36 healthy adults were used in the study. The serum PRA in patients was detected by using the method of complement-dependent micro-lymphocytotoxicity test. Results:Lymphocyte samples from 55 cases of patients were subject to serum lymphocytotoxicity cross matching test,only one case was found positive (1.82%);while by PRA detection serum from 8 cases (6 female and 2 male) showed positive (14.5%). In these patients 35 cases had ever received blood transfusion,of them 6 cases were PRA-positive (17.2%),while of the 20 cases who had never received blood transfusion only 2 cases were PRA-positive (10%). Conclusion:There was difference in the positive rates of PRA between female and male patients ( P <0.05),while no difference was observed between patients having and not having received blood transfusion (P>0.05).Compared with serum lymphocytotoxicity cross test,the detection of PRA is more effective in accurately predicting the in vivo sensitization situation and antibody level in patients.