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miR-218抑制卵巢癌细胞A2780对顺铂耐药性的实验研究 被引量：1

Research of miR-218 inhibit cisplatin resistance of ovarian cancer A2780 cells
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摘要 目的研究miR-218抑制卵巢癌细胞A2780对顺铂耐药性的影响及可能机制。方法实时定量PCR检测A2780及顺铂耐药细胞株A2780/DDP中miR-218的表达,转染miR-218 inhibitors和mimics改变A2780及A2780/DDP细胞中miR-218的表达,MTT法检测转染前后细胞对顺铂的敏感性,并分析Wnt2B蛋白表达的改变。结果与A2780细胞相比,miR-218在A2780/DDP中的表达明显降低。A2780细胞转染miR-218 inhibitors后,细胞对顺铂的敏感性降低,Wnt2B蛋白表达明显增强;而A2780/DDP细胞转染miR-218mimics后,细胞对顺铂的敏感性增强,Wnt2B蛋白表达明显减少。结论 miR-218可能通过靶向Wnt2B抑制卵巢癌细胞A2780对顺铂的耐药性。 Objective To explore the effect of miR-218 inhibit cisplatin resistance of ovarian cancer A2780 cells and mechanism.Methods The expression of miR-218 in A2780 and A2780/DDP cells was detected by real-time PCR. MiR-218 inhibitors and mimics were transiently transfected into A2780 and A2780/DDP ceils respectively.The drug sensitivity of cells to cisplatin and the expression level of Wnt2B were detected by MTT assay and Western blotting. Results The expression of miR-218 in A2780/DDP ceils was significantly decreased compared with A2780 cells. Knockdown of miR-218 dramatically increased proliferative effect induced by cisplatin and expression of Wnt2B in A2780,while overexpression of miR-218 signicantly decreased proliferative effect induction by cisplatin and expression of Wnt2B in A2780/DDP.Conclusion The cisplatin resistance of A2780 ovarian cancer ceils is inhibited by miR-218, and it is maybe by targeting of Wnt2B expression.
作者 饶玉梅 陈彩虹
机构地区 郑州大学第一附属医院妇产科 郑州大学第一附属医院生殖中心
出处 《中国当代医药》 2013年第22期7-9,12,共4页 China Modern Medicine
基金 郑州大学第一附属医院青年创新基金
关键词 MIR-218 卵巢癌 顺铂耐药性 Wnt2B MiR-218 Ovarian cancer Cisplatin resistance Wnt2B
分类号 R737.31 [医药卫生—肿瘤]
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参考文献23

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同被引文献37

  • 1Baohua Li,Ying Hu,Feng Ye,Yang Li,Weiguo Lv,Xing Xie.Reduced miR-34a Expression in Normal Cervical Tissues and Cervical Lesions With High-Risk Human Papillomavirus Infection[J]. International Journal of Gynecological Cancer . 2010 (4)
  • 2Qiaoyan Li,Fufan Zhu,Puxiang Chen.miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer[J].Biochemical and Biophysical Research Communications.2012(1)
  • 3Shuichi Tatarano,Takeshi Chiyomaru,Kazumori Kawakami,Hideki Enokida,Hirofumi Yoshino,Hideo Hidaka,Takeshi Yamasaki,Kazuya Kawahara,Kenryu Nishiyama,Naohiko Seki,Masayuki Nakagawa.miR-218 on the genomic loss region of chromosome 4p15.31 functions asa tumor suppressor in bladder cancer[J].International Journal of Oncology.2011(1)
  • 4Libing Song,Quan Huang,Kun Chen,Liping Liu,Chuyong Lin,Ting Dai,Chunping Yu,Zhiqiang Wu,Jun Li.miR-218 inhibits the invasive ability of glioma cells by direct downregulation of IKK-β[J].Biochemical and Biophysical Research Communications.2010(1)
  • 5CaipingGao,ZhiyuZhang,WenzhongLiu,ShudongXiao,WeiqiGu,HongLu.Reduced microRNA‐218 expression is associated with high nuclear factor kappa B activation in gastric cancer[J].Cancer.2010(1)
  • 6Hang Tian,Lei Hou,Yu-Mei Xiong,Jun-Xiang Huang,Ying-Jun She,Xiao-Bao Bi,Xing-Rong Song.miR-218 suppresses tumor growth and enhances the chemosensitivity ofesophageal squamous cell carcinoma to cisplatin[J]. Oncology Reports . 2015 (2)
  • 7Rong Fan,Jie Zhong,Sichang Zheng,Zhengting Wang,Ying Xu,Shuyi Li,Jie Zhou,Fei Yuan.??MicroRNA-218 inhibits gastrointestinal stromal tumor cell and invasion by targeting KIT(J)Tumor Biology . 2014 (5)
  • 8Qicong Wang,Leyi Wei,Xinjun Guan,Yunfeng Wu,Quan Zou,ZhiLiang Ji.??Briefing in family characteristics of microRNAs and their applications in cancer research(J)BBA - Proteins and Proteomics . 2014 (1)
  • 9Yanyang Tu,Xingchun Gao,Gang Li,Hualin Fu,Daxiang Cui,Hui Liu,Weilin Jin,Yongsheng Zhang.??MicroRNA-218 Inhibits Glioma Invasion, Migration, Proliferation, and Cancer Stem-like Cell Self-Renewal by Targeting the Polycomb Group Gene Bmi1(J)Cancer Research . 2013 (19)
  • 10Takeshi Yamasaki,Naohiko Seki,Hirofumi Yoshino,Toshihiko Itesako,Hideo Hidaka,Yasutoshi Yamada,Shuichi Tatarano,Tomokazu Yonezawa,Takashi Kinoshita,Masayuki Nakagawa,Hideki Enokida.??MicroRNA-218 Inhibits Cell Migration and Invasion in Renal Cell Carcinoma through Targeting Caveolin-2 Involved in Focal Adhesion Pathway(J)The Journal of Urology . 2013 (3)

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中国当代医药
2013年 第22期

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