南方红豆杉悬浮培养细胞团区域化及营养功能分化的研究

Function Differentiation and Compartmentalization of Cell Aggregates in Cell Suspension Culture of Taxus chinensis var.mairei

对南方红豆杉 Taxus chinensis var.mairei细胞悬浮培养过程中的分化、细胞团区域化及营养功能分化等现象进行了研究。通过光学显微镜和电子显微镜对细胞团切片及淀粉颗粒染色从形态学上进行系统观察 ,发现悬浮培养细胞团从外向里呈明显的 3个区域 :表层细胞由成年细胞组成 ,中层细胞是由增殖能力旺盛的细胞组成 ,中心区域的细胞细胞核已消失 ,出现分化的管状分子。在电镜下可见胞间连丝。通过对细胞内淀粉含量及其代谢、可溶性蛋白含量、细胞生物量和总紫杉醇产量的测定结果 ,可以初步判断 3部分区域各自承担着不同的功能 。

The structure of Taxus chinensis var. mairie (Lemée et Lévl.) Cheng et L.K.Fu cell aggregates in suspension culture was studied by observing the section of cell aggregates under optical microscopy.By measuring the content of starch grains, soluble protein, biomass and total taxol, the results showed that there were three distinct compartments: the surface, middle and central layers in cell aggregates. A few plasmodesmas were observed by electron microscopy.The starch grains were mainly distributed in the surface layer and the cells in the middle area showed higher proliferative activity. Preliminary judgement revealed that each of these compartments may have their own different functions, and it was proposed that the nutrient gradient was an important factor for the differentiation of morphology and function of cell compartments in cell aggregates.