摘要
To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.
To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.
