PCNA和p53、c-erbB-2癌基因蛋白在乳腺癌前病变及癌变过程中的相关性

Relationship between PCNA and p53、c-erbB-2 oncogene protein in malignant and premalignant lesions in breast

目的 研究乳腺非典型增生及癌变过程中ＰＣＮＡ及癌基因蛋白ｐ５３、ｃ－ｅｒｂＢ－２的变化规律，并探讨在此过程中细胞增殖活性与ｐ５３、ｃ－ｅｒｂＢ－２癌基因蛋白表达的关系。方法 应用免疫组化法分析了８６例良恶性乳腺组织中的ＰＣＮＡ，癌基因蛋白ｐ５３、ｃ－ｅｒｂＢ－２表达及其相互关系。结果ＰＣＮＡ、ｐ５３、ｃ－ｅｒｂＢ－２的表达随非典型增生程度的增加而增多。ｐ５３、ｃ－ｅｒｂＢ－２阳性表达病例ＰＣＮＣＮ－ＬＩ明显高子阴性病例（ｔ＝２．９０４０，Ｐ＜０．０１；ｔ＝３．２３３３，Ｐ＜０．０１）。结论 ＰＣＮＡ、ｐ５３、ｃ－ｅｒｂＢ－２可反映乳腺癌前病变的恶变倾向，并对癌前病变的临床处理和随访有指导作用；细胞增殖活性与ｒ５３、ｃ－ｅｒｂＢ－２基因激活，蛋白产物聚积有密切联系。

Objective To evaluate cell proliferating activity and expression of P53. c-erbB-2 oncogene protein in breast carcinoma and premalignant lesions in breast and to investigate the relationship of proliferating cell nuclear Aantigen (PCNA) with p53. c-erbB-2 oncogene protein. Methods Immunohistoch emical reaction of PCNA and p53. c-erbB-2 oncogene protein was evaluated in 61 benign lesion and 25 malignant breast paraffinembeded specimens. The relationships of PCNA-Labeling Index (PCNA-LI) and p53 、c-erbB-2 oncogene protein with hyperplasia condition and PCNA-LI with p53、c-erbB-2 oncogene protein were analyzed. Results The expression of PCNA and p53. c-erbB-2 oncogene protein significantly increased with the increased breast atypical hyperplasia condition. PCNA-LI of positive expression for p53 or c-erbB-2 was significantly higher than that of negative respectively (t =2.9040, P <0.01, t =3.2333, P<0. 01). Conclusion The expression of PCNA and p53. c-erbB-2 oncogene protein may indicate the risk of breast premalignant for carcinoma and direct clinical treatment and follow-up for premalignant, and in the meantime the cell proliferating activity had close relation to p53. c-erbB-2 oncogene activation and protein accumulation.