HDL的体外氧化及丙丁酚的抗氧化作用

Oxidation of high-density lipoproteins in vitro and protective effects of probucol

目的 探讨高密度脂蛋白（ＨＤＬ）的体外氧化过程及丙丁酚对ＨＤＬ氧化的抑制作用。方法 采用一次性密度梯度超速离心法获取人血清ＨＤＬ，以ＣＵ～２＋介导其氧化，荧光法检测样品中硫代巴比妥酸反应产物（ＴＢＡＲＳ）的含量，用于判定氧化效果。结果Ｃｕ２＋可有效地介导ＨＤＬ的体外氧化，并在一定范围内呈浓度和时间依赖性；预先加入丙丁酚可有效阻止ＨＤＬ的体外氧化，５μｍｏｌ／Ｌ丙丁酚即有显著的抗氧化作用。结论 ＨＤＬ亦易于发生氧化修饰，抗氧化剂丙丁酚可明显抑制由Ｃｕ２＋介导的ＨＤＬ体外氧化。

Objective To investigate the oxidation of high-density lipoproteins(HDL) in vitro and protective effects of probucol. Methods Human plasma lipoproteins were separated by one-step ultracentrifugation with a simple density-gradient. The in vitro oxidation of HDL was mediate by CuSO4, the oxidative effect of Cu2+ and the antioxidation effect of probucol were assayed simultaneously by thiobarbituric acid reactive substances (TBARS). Results (1) Cu2+ could effectively mediated the oxidation of HDL in vitro, which was both dose-dependent and time-dependent with Cu2+ within definite ranges.(2) The oxidation of HDL in vitro could be inhibited by probucol, and 5μmol/L probucol was effective obviously. Conclusion HDL is susceptible to oxidation and probucol can inhibit the Cu2+ mediated oxidation of HDL in vitro.