心肌缺血预处理对心肌的保护作用

The protective role of ischemic preconditioning in Wistar rat heart

目的 探讨心肌缺血预处理（ＩＰＣ）保护该心肌被以后较长时间缺血损伤的作用。方法 给Ｗｉｓｔａｒ大鼠在９０ｍｉｎ缺血和１８０ｍｉｎ再灌注（Ｉ／Ｒ）前３次、每次５ｍｉｎ、间隔５ｍｉｎ再灌注的心肌缺血预处理；Ｉ／Ｒ前静脉注射Ｂｒｅ．、ＰＭＡ观察它们对心肌Ｉ／Ｒ损伤的影响作用。结果 ＩＰＣ、ＰＭＡ可保护心肌被Ｉ／Ｒ的损伤；Ｂｒｅ．则可消除ＩＰＣ对心肌的保护作用。结论 ＩＰＣ可保护心肌被长达９０ｍｉｎ．Ｉ／１８０ｍｉｎ．Ｒ损伤，其保护机制作者正在研究之中，可能与蛋白激酶Ｃ被激活有关。

Objective To explore IPC(ischemic preconditioning) and PMA reduce the size of infarcts compared with control and breviscapini(Bre.). Methods and Results Anesthetized open-chest wistar rats underwent three 5- minute episodes of preconditioning ischemia in IPC group or after administration of Bre. in Bre.group; Control group had no episodes of ischemic preconditioning; PMA was injected 5 minutes before sustained occlusion; Afte- r 90 minutes of CAO and 3 hours of reperfusion, infarct size was significantly reduced in IPC rats' compared with control group and Bre.+IPC groups, P<0.01. Conclusion The wistar rats IPC model was stable, TTC can obviously distinguish the AN(area of necrosis) from normal myocardium. The IPC、PMA protected the myocardium from sustained ischemia-reperfusion injury.Bre. blunted the protective role of IPC. PMA imitated the protective role of IPC. Their protective mechanism will be disscused in detail in next article.