端粒保护蛋白1在不同证型获得性再生障碍性贫血患者中的表达及相关性研究

POT1 Gene Expression in Patients of Acquired Aplastic Anemia with Different TCM Syndromes and Correlation Study

目的观察端粒保护蛋白1(POT1)在不同证型获得性再生障碍性贫血(再障)患者外周血单个核细胞的表达水平,探讨其与获得性再障及其中医辨证分型之间的关系。方法采用实时荧光定量聚合酶链反应(RT-qPCR)检测52例不同证型获得性再障患者和20例正常对照组外周血单个核细胞中POT1 mRNA表达情况,并进行相关性分析。结果获得性再障患者POT1 mRNA表达情况较正常对照组明显减低(P<0.05)。肾阴虚型POT1 mRNA表达水平低于肾阳虚型,肾阴阳两虚型最低,并且与年龄有明显相关性(r=0.374,P=0.038)。结论 POT1参与了获得性再障的发病经过,其在外周血单个核细胞的表达水平与获得性再障的中医证型有一定相关性。

Objective To observe the mRNA expression levels of POT1 in peripheral blood mononuclear cells of acquired aplastic anemia patients with different syndrome,and explore its relationship with acquired aplastic anemia and its TCM syndrome.Methods Peripheral blood mononuclear cells of 52 cases with acquired aplastic anemia and 20 cases as control group were collected to detect mRNA expression of POT1 by using real-time quantitative polymerase chain reaction（RT-qPCR）,and its relation with TCM syndrome was analyzed.Results The expression levels of POT1 mRNA in patients with acquired aplastic anemia were lower significantly than control group（P〈0.05）.The expression levels of POT1 mRNA in patients with deficiency of kidney-yin were lower than patients with deficiency of kidney-yang,and it was lowest in patients with kidney deficiency of both yin and yang.There was significant correlation between the expression levels of POT1 mRNA and age（r =0.374,P =0.038）.Conclusion The changes in expression levels of POT1 play a role in the pathogenesis of acquired aplastic anemia.There is correlation between mRNA expression level of POT1 in peripheral blood mononuclear cells and TCM syndrome.