红芪水提浓缩液对快速老化小鼠学习记忆和单胺类神经递质的影响

Influence of Hedysari Radix Concentrations Fluid on Learning and Memory and Monoamine Neurotransmitters in Senescence Accelerated Mice

目的观察红芪水提浓缩液(HRCF)对快速老化小鼠学习记忆能力的改善作用和对脑组织内单胺类神经递质的影响,探讨其改善小鼠学习和记忆能力的作用机制。方法将老化小鼠随机分为抗快速老化亚系1(SAMR1)对照组、快速老化亚系8(SAMP8)模型组、HRCF组和安理申组,每组20只,持续灌胃给药3个月。给药结束后,通过Morris水迷宫测试各组小鼠学习和记忆成绩的变化,采用高效液相色谱法检测各组小鼠脑组织中去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)及5-羟吲哚乙酸(5-HIAA)含量的变化。结果模型组快速老化小鼠隐蔽平台的逃避潜伏期明显延长,脑组织中NE、DA、5-HT及5-HIAA含量显著降低(P<0.01)。与模型组比较,HRCF组小鼠隐蔽平台的逃避潜伏期明显缩短,脑组织中NE、DA、5-HT及5-HIAA含量明显增加,差异有统计学意义(P<0.05,P<0.01)。结论 HRCF能够改善痴呆模型小鼠的学习和记忆能力,其机制可能与调节脑组织内单胺类神经递质的含量有关。

Objective To explore the influence of Hedysari radix concentrations fluid（HRCF） on learning and memory and its effect on cerebral monoamine neurotransmitters in senescence accelerated mice.Methods The senescence accelerated mice were randomly divided into the senescence accelerated mouse/resistance 1（SAMR1） control group,senescence accelerated mouse/prone-8（SAMP8） model group,HRCF treatment group and aricept treatment group.After 3 months of intragastrical treatment,the learning and memory ability changes and content of norepinephrine,dopamine,5-hydroxytryptamine and 5-hydroxyindoleacetic acid were detected respectively using Morris water maze and high performance liquid chromatography.Results In the model group,the hidden platform test indicated that the latency to find the hidden platform was remarkably prolonged,and the content of norepinephrine,dopamine,5-hydroxytryptamine as well as 5-hydroxyindoleacetic acid were significantly reduced（P〈0.01）.Compared with the model group,the latency of hidden platform test was remarkably shortened（P〈0.05） and the content of norepinephrine,dopamine,5-hydroxytryptamine as well as 5-hydroxyindoleacetic acid were obviously increased in HRCF treatment group（P〈0.01）.Conclusion HRCF could improve learning and memory ability of SAMP8 and the influence may related to increasing the content of cerebral monoamine neurotransmitters.