摘要
目的探讨应用胰岛素生长因子1(IGF-1)对于动脉粥样硬化斑块平滑肌细胞的影响,为其应用于控制斑块易损性提供理论依据。方法雄性新西兰大白兔18只,随机分为3组,每组6只,分别为模型组、IGF-1干预组、辛伐他汀干预组。制作动脉粥样硬化模型。处死后解剖分离腹主动脉斑块标本,组织染色测量斑块纤维帽厚度及纤维帽的厚度与脂核横截面厚度的比值;免疫组化测量斑块平滑肌细胞含量,进行统计学分析。结果 IGF-1组与模型组比较,在斑块肩部及顶部,两者差异有统计学意义;辛伐他汀组与模型组比较,二者差异有统计学意义,辛伐他汀组与IGF-1组,两者帽核比均大于模型组帽核比。而IGF-1组与辛伐他汀组比较,二者差异无统计学意义。两干预组平滑肌细胞含量均与模型组有统计学差异,而辛伐他汀组平滑肌细胞含量增多趋势最明显。结论应用IGF-1可以诱导平滑肌细胞增殖,影响斑块的组织机构,使易损斑块的纤维帽厚度得到加强,从而使斑块破裂的可能性下降,达到稳定斑块的目的。
Objective To explore the application of IGF 1 for smooth muscle cells of atherosclerotic plaque for its application to control of plaque vulnerability and provide a theoretical basis. Methods 18 male new Zealand white rab- bits were made the atherosclerotic model. The rabbits were euthanized. The entire length of the aorta was exposed and was divided several segment~. Then histological assessment of experimental atherosclerosis,immunohistochemical stai- ning for smooth muscle cell were performed. At last,statistical analysis was made. Results To compare the group of IGF-1, simvastatin and model,the differences on top fiber cap thickness, shoulder fiber cap thickness, the ratio of fiber cap vs lipid core were statistical significance. However, the differences between IGF-1 and simvastatin group were non--statistics significance. The smooth muscle cells of two intervention groups were compared with the model group, there was statistically significant difference,while the simvastatin group smooth muscle cell content increasing trend is the most obvious. Conclusion The present study demonstrates IGF-lcould induce smooth muscle cell proliferation,the vulnerable plaque fibrous cap thickness has been strengthened,And thus stabilizing the atherosclerotic plaque.
出处
《中国实验诊断学》
2013年第7期1173-1175,共3页
Chinese Journal of Laboratory Diagnosis
