MSCs对辐射诱导小鼠胸腺瘤中T细胞CD4/CD8各亚群及各亚群中CD45比例变化的影响

The effect of MSCs on change of each CD4/CD8 Tcell subsets in the proportion of all cells and CD45 in the proportion of each subgroup of thymoma induced by radiation

目的观察MSCs对辐射诱导小鼠胸腺瘤中T细胞CD4/CD8各亚群及各亚群中CD45比例变化的影响,探讨MSCs对辐射诱发肿瘤的预防和治疗作用。方法应用Kaplan经典方法制备C57BL/6幼年鼠胸腺瘤模型[1],经小鼠尾静脉输注同系鼠MSCs,6个月后取胸腺组织,将胸腺细胞制成单细胞,标记CD4、CD8、CD45,流式细胞术分析T细胞亚群。结果流式细胞术分析结果显示:照射后CD4-CD8-亚群比例由正常组的(5.11±5.10)%降低到(0.01±0.01)%(P<0.05),MSCs治疗(0.61±0.39)%后有所恢复;MSCs治疗组(91.49±4.47)%中CD4+CD8+亚群比例与正常组(82.44±6.06)%接近,单纯照射组(99.09±0.49)%显著高于正常组(P<0.05);照射后CD4+CD8-亚群由(7.72±3.37)%下降到(0.87±0.49)%,CD4-CD8+亚群由(4.74±2.59)%下降到(0.02±0.01)%,MSCs治疗后CD4+CD8-和CD4-CD8+都有不同程度上调。正常组、单纯照射组、MSCs治疗组中CD45+在CD4+CD8+中的比例分别为(86.09±2.24)%、(0.16±0.03)%、(97.35±3.23)%,差异有统计学意义(P<0.05);单纯照射组CD4-CD8-、CD4-CD8+和CD4+CD8-中CD45+均有不同程度的降低,MSCs治疗组有不同程度升高,且具有统计学意义(P<0.05)。结论 MSCs可使辐射诱导胸腺瘤模型组小鼠胸腺中T细胞CD4/CD8各亚群及各亚群中CD45比例增高或恢复,提示MSCs具有防治辐射诱发胸腺瘤的作用,并对辐射造成的胸腺损伤具有修复作用。

Objective Each CD4/CD8 T cell subsets in the proportion of all cells and CD45 in the proportion of each subgroup were observed ,to investigate the prevention and treatment of MSCs to radiation-induced thymoma. Methods Kaplan classic method was applied to prepared C57BL/6 young mouse thymoma modelEI？ ,and fellow rat＇s MSCs was injected via the tail vein,after 6 months we get the thymus tissue,then make the thymus cells to single cells and marked CD4,CD8 and CD45,and flow cytometry was used to analyze T cell subsets. Results Flow cytometry results showed that after irradiation,CD4 CD8 subsets decreased from the normal group （5.11-＋-5.10） ~ to （0.01--t-_0.01） ~ （P-Q0. 05） ,after the treatment of MSCs（0.61--0. 39）~//00, it has restored; CD4＋ CD8＋ subsets in group of MSCs＇ treatment （91.49：t：4.47）~ was closed to the normal group（82.44q-6.06） ~,irradiation group （99.09~0.49）~ was signifi- cantly higher than the normal group（P^0.05） ;after irradiation,CD4＋ CD8 subsets decreased from the normal group （7.72-1-3.37） ~/00 to（0.87-1-0.49）＋ ,CD4-CD8＋ subsets decreased from （4.74±2.59）% to（0.02±0.01）% ,after the treatment of MSCs,CD4＋ CD8 and CD4 CD8＋ increased in different degrees. CD45＋ in the proportion of CD4＋ CD8＋ in normal group,irradiation group and treatment group respectively was （86.09 ± 2.24）%, （0.16± 0.03） %, （97.35 ± 3.23）% ,the differences were ~ignifieant（P〈0.05） ;CD45＋ in the proportion of CD4 CD8 ,CD4 CD8＋ and CD4＋ CD8 in irradiation group decreased in different degrees,after the treatment of MSCs,they increased in different degrees, the differences were significant （P〈0.05）. Conclusion MSCs can make each CD4/CD8 T cell subsets in the proportion of all cells and CD45 in the proportion of each subgroup increase or restore,it shows that MSCs have prevention and treat- ment to radiation-induced thymoma,and have repair to the thymus injury that was caused by radiation.