摘要
运用聚合酶链反应 (PCR)技术 ,结合短串联重复序列 (STR)多态性标记的方法 ,对山东地区汉族 10 7例 2型糖尿病患者和 10 5例正常对照者 ,进行葡萄糖激酶 (GCK)基因及小肠脂肪酸结合蛋白 (FABP2 )基因的多态性分析。结果显示 GCK等位基因 A5与 2型糖尿病组呈正相关 (RR=15 .70 ,P<0 .0 0 1) ;GCK等位基因 A1、A3与 2型糖尿病组呈负相关 (RR=0 .2 8,P<0 .0 0 1;RR=0 .15 ,P<0 .0 0 1)。FABP2各等位基因频率在两组分布未见显著性差异。多因素非条件 L ogistic回归分析发现 ,2型糖尿病的发病与 GCK等位基因 A5呈显著正相关 ;与 GCK等位基因 A3呈显著负相关。年龄、甘油三酯、载脂蛋白 B及脂蛋白 a亦是 2型糖尿病发病的危险因素。认为 GCK基因多态性与山东地区汉族 2型糖尿病具有相关性 ,是 2型糖尿病的危险因素。FABP2 基因与 2型糖尿病未见相关性。
Two STR region polymorphism were analyzed with the polymerase chain reaction and polyacrylamide sequencing gels in 107 subjects with type 2 diabetes mellitus and 105 nondiabetic subjects All subjects were biologically unrelated Chinese living Shandong without family history Results showed 5 alleles and 9 genotypes of GCK gene locus ,4 alleles 7 genotypes of FABP 2 gene locus were observed The differences in allelic frequencies of GCK gene between individuals with type 2 diabetes and nondiabetic individuals were compared(p<0 001) No difference was found in the frequency of FABP 2 alleles between the two groups(p<0 05) A5 allele of GCK gene was associated with type 2 diabetes (RR=15 70,p<0 001) Logistic regression analysis showed A5 allele was associated with type 2 diabetes We conclude ploymorphic microastellite repeat markers at the glucokinase gene locus is positively associated with type 2 diabetes in Chinese The polymorphism at the FABP 2 locus is not genetic marker for type 2 diabetes in Chinese
出处
《山东医药》
CAS
北大核心
2000年第14期13-14,共2页
Shandong Medical Journal
基金
国家自然科学基金!资助项目 (批准号 395 0 0 72 )
