PTEN survivin基因在肝癌组织中的蛋白表达及相关研究

The Expression of PTEN and Survivin in the Hepatocellular Carcinoma

目的:应用免疫组化的方法研究肝细胞肝癌组织中抑癌基因PTEN,癌基因survivin蛋白的表达情况以及与临床病理学特征的关系,探讨其间相互关系,为相关的基因治疗的研究提供依据。方法:43例HCC标本及癌旁肝组织标本,临床资料和病理资料完整,经石蜡包埋保存。基因的染色方法均为免疫组织化学S-P法,进行光镜观察和统计学分析。结果:43例HCC组织中PTEN,sur-vivin蛋白表达阳性率分别为48.8%、69.77%,癌旁组织为100%,11.8%。PTEN的表达分别与血中AFP浓度和组织病理分化程度(Edmondson分级)有关;survivin的表达与血中AFP及HCC Edmondson病理分化程度相关,survivin与肿瘤的侵袭危险度相关。spearman相关分析发现HCC组织中PTEN与survivin的表达具有相关(r=-0.471,P=0.031;)。结论:PTEN和survivin的蛋白表达呈负相关,二者均参与HCC的发病过程,共同调控肝脏组织恶变的病理过程。

Objective： To explore the expression of anti-oncogene PTEN and oncogene survivin in the hepatocellular carcinoma （ HCC ） , and the relationship with clinical pathology, provide the basis for the related gene treatment research. Method ：43 specimens of HCC were obtained from the operative specimens. The PTEN and survivin were measured by the immunohistochemistry S-P methods, carried on the microscope observation and statistics analysis. Result： The positive expression rate of PTEN and survivin in HCC was 48.8% and 69.77% respectively, paracarcinoma tissue was 100% and 11.8%. The positive expression of FFEN was relative to blood AFP concentration and Edmondson differ degree. The positive expression of sur- vivin is also relative to blood AFP concentration and Edmondson differ degree. The expression of survivin is relative to tumor invasion danger degree . By spearman relative analysis, There＇s significant correlation between the positive expression of PTEN and survivin （r= -0.471, P=0.031）. Conclusion：PTEN and survivin has negative regression. The two genes maybe joinin and regulate coordinated the deteriorate pathological progress of liver tissue together.