腺病毒载体介导的自杀基因对MFC小鼠前胃癌细胞的体外杀伤作用

The in vitro Killing Effect of Suicide Gene Delivered by Adenovirus Vector on MFC Gastric Cancer Cells

提高转基因效率，建立高效自杀基因杀伤胃癌肿瘤细胞的方法。方法：首先构建含CD基因的重组腺病毒载体并对载体进行包装、收获病毒上清并纯化。将重组病毒液转染MFC胃癌细胞进行体外抑瘤实验。结果：含CD基因的重组腺病毒载体经酶切鉴定选出：包装纯化后，检测病毒浓度为1.2×1012颗粒/ml。体外实验中，转CD基因的MFC细胞在5-FC作用下生长明显受抑(P<0.01,n＝9)。结论：腺病毒载体转导的CD基因提高了转染效率，为大量杀伤胃癌细胞奠定了基础。

To improve the efficacy of gene delivery and to establish an effective mode of killing gastric cancer cells by means of CD suicide gene. Medthods The CD gene-containing recombinant adenovirus vector was cloned, packaged, amplified and purified. The MFC gastric cancer cells were transfected by the adenovirus supernatant, and were then subjected to 5-FC treatment for 5 days. Results：Positive clones were selected by using the endonuclease method and the concentration of CD gene-containg viral super-natant was 1.2×1012 particles/ml. Inhibition of MFC cells was observed when 5-FC was given. Conclusions: The anti-tumor effect of CD gene enhanced by delivery via adenovirus vector could serve as a basis for further researches in the use of surcide genes in the treatment of gastric carcinoma.