粘附分子P选择素单抗对人胃癌荷瘤鼠免疫功能的影响

Effect of P-selectin Monoclonal Antibody on Immune Function of SCID Mice Orthotopically Implanted with Human Gastric Cancer Tissue

研究P选择素单克隆抗体对SCID小鼠免疫功能的影响。方法：用人胃癌组织块原位移植于SCID小鼠，制作转移模型。术后第3天开始，分别静脉注射P选择素单抗(肿瘤处理组)，每次100μg，每周2次，共3周，或PBS(肿瘤组)，每次100μl，每周2次，共3周。此外，正常组分为正常处理组与正常对照组，分别静脉注射P选择素单抗或PBS，剂量与方法同上。分别于术后第一、二、四周随机处死2只小鼠，其余均在第六周末处死。取胃癌组织、肿大淋巴结和可疑转移脏器作病理学检查；无菌取脾脏测定免疫功能。用3H-TdR掺入法测定T、B细胞转化指数，LDH释放法测定NK杀伤活性。结果：肿瘤处理组转移率低于肿瘤组。正常SCID小鼠NK活性随鼠龄增大而升高；肿瘤组第六周的免疫功能明显低于第一、二、四周，肿瘤处理组T、B细胞转化指数和NK活性在第六周时明显高于肿瘤组(均P<0.05)。结论：P选择素单抗对肿瘤转移具有抑制作用，而对小鼠细胞免疫功能无明显抑制作用。

o investigate the effect of cell adhesion molecule P-selectin monoclonal antibody(Mab) on the immune function of mice orthototopically implanted with human gastric cancer tissue.Methods SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3, animals were given intravenously PBS or P-selectin Mab(100μg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the 3H TdR infiltration method, the NK cell activity was detected by the LDH release method. Results The metastatic rate as lower in the P-selectin Mab treated gorup than in the PBS treated group(with tumors). The NK activity of normal mice mounted higher with the passage of time. The immune functions(T, B cell function, NK activity) of the tumor group were significantly lower in the 6th week than in the 4th week, but the change was attenuated by P-selectin Mab. Conclusions: P-selectin Mab could inhibit the metastatic tendency with no harmful effect on host immune function.