鸽粪中新生隐球菌的分离和鉴定

Isolation and identification of Cryptococcus neoformans from pigeon dropping

采用GAEC培养基培养了144份私人和公共养鸽场所的鸽粪标本，从中共分离得32株新生隐球菌，阳性率达22.2％。37℃生长良好。尿素试验均为阳性。血清因子分型结果证实阳性标本均为新生隐球菌新生变种，全是A血清型，动物接种后能重新分离出该菌。病理证实具致病性。

Thirty two Cryptococcus neoformans strains were isolated from 144 samples of pigeon droppings in private and public square pigeon shelters in Shanghai on GAEC. The positive isolation rate was 22.2％ (32/144). All strains are Cry. neoformans var. neoformans and serotype A identified by Crypto. Cheek Kit(Iatron laboratories Inc Tokyo, Japan). Also, Christensen's Urea testes were all positive. Mice were inoculated by two strains in abdomen and same strains can be isolated again. All strains can grow well at 37℃ . All above showed that these strains have pathogenicity.