摘要
【目的】探索SD大鼠孕哺期给予高能量饲料对子代脂代谢的影响。【方法】成年SD孕鼠15只随机分为对照组、高脂组、高脂高糖组,在孕哺期分别给予标准鼠粮、高脂饲料(含20%猪油)和高脂高糖饲料(含20%猪油和10%蔗糖)。仔鼠4周处死,称量体重、肝脏和肾周脂肪质量,并检测血浆甘油三酯、胆固醇、高密度脂蛋白和瘦素水平。【结果】体重、肝脏和肾周脂肪质量,高脂组[(90.05±6.71)、(3.73±0.18)和(0.41±0.12)g]和高脂高糖组[(89.82±5.08)、(3.82±0.27)和(0.41±0.13)g]均高于对照组[(70.60±17.30)、(2.87±0.83)和(0.22±0.07)g](P<0.05);高脂高糖组总胆固醇水平(1.72±0.10)高于高脂组(0.9±0.33)和对照组(0.8±0.3)(P<0.05);高脂组(0.83±0.54)和高脂高糖组(1.02±0.22)的甘油三脂水平高于对照组(0.45±0.21)(P<0.05);高密度脂蛋白和瘦素,高脂组(0.23±0.08,1.16±0.06)和高脂高糖组(0.26±0.09,1.15±0.04)的低于对照组(0.50±0.03,1.26±0.08)(P<0.05)。【结论】SD大鼠孕哺期摄入高能量饲料造成子代肥胖、血脂异常和血浆瘦素水平下降等脂代谢紊乱。
[Objective] To explore the effect of perinatal high energy on lipid metabolism in SD rat offspring. [Methods] A total of 15 pregnant SD rats were divided randomly into control group,high fat group and high fat and high carbohydrate group,which were fed with lab chow,high fat diet(containing 20 % of lard) and high fat and high carbohydrate diet(containing 20% of lard and 20% of sucrose) respectively. The pups of 4 weeks old were killed,and their body weight, the mass of liver and viscera] fat and the levels of plasma TC,TG,HDL and leptin were determined. [Results] With as- pects of body weight,the mass of liver and visceral fat,the data of high fat group(90.05±6.71) ,(3.73±0.18) and (0.41±0.12)g and high fat and high carbohydrate(89.82±5.08), (3.82±0.27) and (0.41±0.13)g were higher than those of control group(70.60±17.30) ,(2.87±0.83) and (0.22±0.07)g (P〈0.05) ;The level of plasma TC of high fat and high carbohydrate(1.72±0.10) was higher than that of high fat group(0.9±0.33) and control group(0.8±0.3)(P〈0.05); The levels of plasma TG of high fat group (0.83±0.54) and high fat and high carbohydrate group(1.02±0.22) were higer than that of contro[ group(0.45±0.21) (P〈0.05) ;The level of plasma HDL and [eptin of high fat group(0.23 ± 0.08), (l. 16±0.06) and high fat and high carbohydrate group(0.26±0.09), (1.15±0.04) were higher than those of control group(0.50±0.03), (1.26±0.08)(P〈0.05). [Conclusion] Perinatal high energy leads lipid dysfunction including obe- sity,disorder of plasma lipid,decline of plasma leptin and so on.
出处
《中国儿童保健杂志》
CAS
北大核心
2013年第8期815-816,819,共3页
Chinese Journal of Child Health Care