SD大鼠孕哺期喂养高能量饲料对子代脂代谢的影响

Effect on perinatal high energy diet on lipid metabolism in childhood

【目的】探索SD大鼠孕哺期给予高能量饲料对子代脂代谢的影响。【方法】成年SD孕鼠15只随机分为对照组、高脂组、高脂高糖组,在孕哺期分别给予标准鼠粮、高脂饲料(含20%猪油)和高脂高糖饲料(含20%猪油和10%蔗糖)。仔鼠4周处死,称量体重、肝脏和肾周脂肪质量,并检测血浆甘油三酯、胆固醇、高密度脂蛋白和瘦素水平。【结果】体重、肝脏和肾周脂肪质量,高脂组[(90.05±6.71)、(3.73±0.18)和(0.41±0.12)g]和高脂高糖组[(89.82±5.08)、(3.82±0.27)和(0.41±0.13)g]均高于对照组[(70.60±17.30)、(2.87±0.83)和(0.22±0.07)g](P<0.05);高脂高糖组总胆固醇水平(1.72±0.10)高于高脂组(0.9±0.33)和对照组(0.8±0.3)(P<0.05);高脂组(0.83±0.54)和高脂高糖组(1.02±0.22)的甘油三脂水平高于对照组(0.45±0.21)(P<0.05);高密度脂蛋白和瘦素,高脂组(0.23±0.08,1.16±0.06)和高脂高糖组(0.26±0.09,1.15±0.04)的低于对照组(0.50±0.03,1.26±0.08)(P<0.05)。【结论】SD大鼠孕哺期摄入高能量饲料造成子代肥胖、血脂异常和血浆瘦素水平下降等脂代谢紊乱。

[Objective] To explore the effect of perinatal high energy on lipid metabolism in SD rat offspring. [Methods] A total of 15 pregnant SD rats were divided randomly into control group,high fat group and high fat and high carbohydrate group,which were fed with lab chow,high fat diet（containing 20 % of lard） and high fat and high carbohydrate diet（containing 20% of lard and 20% of sucrose） respectively. The pups of 4 weeks old were killed,and their body weight, the mass of liver and viscera] fat and the levels of plasma TC,TG,HDL and leptin were determined. [Results] With as- pects of body weight,the mass of liver and visceral fat,the data of high fat group（90.05±6.71） ,（3.73±0.18） and （0.41±0.12）g and high fat and high carbohydrate（89.82±5.08）, （3.82±0.27） and （0.41±0.13）g were higher than those of control group（70.60±17.30） ,（2.87±0.83） and （0.22±0.07）g （P〈0.05） ;The level of plasma TC of high fat and high carbohydrate（1.72±0.10） was higher than that of high fat group（0.9±0.33） and control group（0.8±0.3）（P〈0.05）; The levels of plasma TG of high fat group （0.83±0.54） and high fat and high carbohydrate group（1.02±0.22） were higer than that of contro[ group（0.45±0.21） （P〈0.05） ;The level of plasma HDL and [eptin of high fat group（0.23 ± 0.08）, （l. 16±0.06） and high fat and high carbohydrate group（0.26±0.09）, （1.15±0.04） were higher than those of control group（0.50±0.03）, （1.26±0.08）（P〈0.05）. [Conclusion] Perinatal high energy leads lipid dysfunction including obe- sity,disorder of plasma lipid,decline of plasma leptin and so on.