血清肿瘤标志物联合检测在肺癌诊断中的临床应用

Clinical application of combined detection of serum tumor markers in diagnosis of lung cancer

目的探讨血清肿瘤标志物联合检测在肺癌诊断中的意义，并选出敏感性最高的血清肿瘤标志物组合。方法应用酶联免疫吸附法检测62例肺癌患者和62例健康体检者血清中神经元特异性烯醇化酶（NES）、糖链抗原125（CAl25）、细胞角蛋白19（CYFRA21-1）及癌胚抗原（CEA）的含量水平。结果肺癌组患者血清CEA、CAl25、CYFRA21-1及NES水平均高于对照组（均P〈0．05）；在4种肿瘤标志物中，CAl25的敏感性最高（59．7％），其次为CEA（53．2％）；4种标志物联合检测的敏感性为87．1％，3种标志物联合检测中，以CEA＋CAl25＋CYFRA21-1敏感性最高，为85．5％，2种标志物联合检测中CEA＋CAl25敏感性最高，为75．8％。结论4种血清肿瘤标志物对于肺癌的辅助诊断有一定的临床意义，CEA、CAl25、CYFRA21．1可作为联合检测肺癌的首选标志物，具有较高的敏感性。

Objective To investigate the significance of combined detection of serum tumor markers in the diagnosis of lung cancer,and to select the most sensitive serum tumor markers. Methods Enzyme-linked immunosor- bent assay was used to detect the serum tumor markers of 62 lung cancer patients and 62 healthy persons, who were neuron specific enolase enzyme（ NES）, carbohydrate antigen 125 （ CA125 ）, cytokeratin 19 （ CYFRA21-1 ） and carci- noembryonic antigen（CEA）. Results The level of serum CEA, CA125, CYFRA21-1, and NES of the lung cancer group were all higher than those of the control group （all P 〈0.05）. Among the four kinds of tumor markers,CA125 had the highest sensitivity （59.7%）, which was followed by CEA （53.2%）. The sensitivity of the four kinds of joint detection was 87. 1%, the highest sensitivity of three markers joint detection was CEA ＋ CA125 ＋ CYFRA21-1 （87. 1% ） ,while that of the two kinds of joint detection was CEA ＋ CA125 （75.8%）. Conclusion There are high clinical significance in the diagnosis of lung cancer with four kinds of serum tumor markers, CEA ＋ CA125 ＋ CYFRA21-1 can be used as the best markers for joint detection of lung cancer with high sensitivity.