罗汉果叶黄酮对力竭大鼠组织细胞膜保护作用研究

Protection of Flavonoids Extracted from Siraitia grosvenorii Leaves to Different Tissue Membranes in Rats During Exhaustive Exercise

为研究罗汉果叶黄酮提取物抗氧化损伤的保护作用,为罗汉果叶资源的开发利用提供理论依据,选用健康雄性SD大鼠40只,2～3月龄,体质量180～220g,随机分成5组,每组8只,即:安静对照组、安静力竭组、给药力竭组、训练力竭组、训练给药力竭组。给药量以生理盐水配成含罗汉果叶黄酮提取物20g/L的溶液,给药组按10.0mL/(kg.d-1)灌胃给药,对照组灌服同等剂量的生理盐水。在递增负荷游泳训练28d后,各组进行3%的负重力竭性游泳训练,准确记录力竭时间后即刻断头取血(安静对照组同时取血),测定血浆、心脏、肝脏和股四头肌的GOT、GPT和CK指标,并且在光学显微镜下观察心脏、肝脏和股四头肌病理组织学变化。结果表明,灌注罗汉果叶黄酮提取物可分别提高游泳至力竭后大鼠心肌、肝脏及股四头肌细胞特异酶GOT、GPT、CK的活性,降低血浆中相应酶的活性;通过光学显微镜对组织切片观察,发现服用罗汉果叶黄酮提取物的大鼠心肌、肝脏及股四头肌细胞膜均比未服药的对照组完整,发生破裂、变形的比例大大降低,说明罗汉果叶黄酮提取物对心肌、肝脏及股四头肌细胞膜具有较好的保护作用,在一定程度上保证了细胞膜的完整性及流动性。

To provide a theoretic evidence for exploitation and application of Siraitia grosvenorii leaves resources,the protection effect of flavonoids extracted from Siraitia grosvenorii leaves in response to oxidative stress injury was studied.Forty healthy male Sprague-Dawley rats（body weigh of 180-220 g,aged 2-3 months） were randomized into five groups,each consisting of 8 rats：the quiet control group,the pro-quiet exhaustive group,the pro-administration exhaustive group,the pro-training exhaustive group and the pro-administration-training exhaustive group.Drug-treated groups were administrated（i.g.） the solution of physiological saline paired with flavonoids extracted from Siraitia grosvenorii leaves（20 g/L） at a dose of 10.0 mL/kg body weight per day;while other groups were administrated of pure physiological saline at the same dose.After the incremental swimming training of 28 d,rats in exhaustion groups were bore their own weight by 3% and swam to exhaustion.Rats（including the rats in quiet control group） were killed by decapitation immediately after accurately recorded the exhaustive time.Blood,heart,liver and quadriceps tissues were sampled for subsequent analysis of enzymes＇ activities（GOT,GPT and CK）,and observation of histopathological changes with microscope.The results showed that flavonoids extracted from Siraitia grosvenorii leaves can obviously increase the activities of GOT,GPT and CK in heart,liver and quadriceps tissues,decrease these corresponding enzymes＇ activities in plasma.The cell membranes of heart,liver and quadriceps tissues in drug-treated groups are much more integrated than that of non drug-treated groups.The ratios of rupture and deformation are greatly reduced in drug-treated groups.The conclusion indicates that flavonoids extracted from Siraitia grosvenorii leaves can help to protect the cell membranes of heart,liver and quadriceps tissues,ensure the integrity and fluidity of the membranes.