美洲大蠊提取物含药血清体外抗肝纤维化的实验研究

Anti-hepatic Fibrosis Effects Research on Serum Containing Extracts of Periplaneta americana in vitro

目的:研究美洲大蠊提取物含药血清对大鼠肝星状细胞的影响。方法:通过制备美洲大蠊提取物含药血清,MTT法观察美洲大蠊提取物含药血清对肝星状细胞的作用,酶联免疫吸附剂测定法(Elisa)检测培养上清中TGF-β1、Ⅰ型胶原的含量。结果:24、48、72 h美洲大蠊提取物Ⅰ(水提物)、Ⅱ(乙醇提取物)含药血清(15%)对肝星状细胞均有抑制作用(P!0.05);美洲大蠊提取物Ⅰ、Ⅱ含药血清在24、48 h能减少Ⅰ型胶原的生成,但无统计学差异(P"0.05);24 h美洲大蠊提取物Ⅰ含药血清15%、9%、5.4%均能减少TGF-β1的生成(P!0.05),48 h时,只有15%含药血清能减少TGF-β1的生成(P!0.05),美洲大蠊提取物Ⅱ含药血清在24、48 h时未显著减少TGF-β1的生成(P"0.05)。结论:美洲大蠊提取物含药血清具有一定的体外抗肝纤维化作用,其机制可能为抑制肝星状细胞的增殖及减少TGF-β1的生成。

Objective ： To study the anti-hepatic fibrosis effect of serum containing extracts of Periplaneta americana. Methods ： The serum contained extracts of Periplaneta americana was prepared with serologic pharmacological method. ~,＇ITY method was used to ob-serve the effect of serum containing extracts from periplaneta americana on hepatic stellate cells（HSC） ,and Elisa method was used to detect the contents of TGF-β1 and collagen I in supernatant. Results： Serum containing extracts Ⅰ and Ⅱ （15%） of Periplaneta ameri-cana had inhibitou effect on HCS （ P 〈 0.05 ） after HSC were cultured with serum containing extracts of different concentration of Perio-laneta americana for 24,48 and 72 h. At 24 and 48 h, serum containing extracts Ⅰ and Ⅱ of Periplaneta americana decreased the con-tent of collagen l in supernatant without slgnifieant difference （P 〈 O. 05 ）. Serum containing extracts Ⅰ （ 1 5% ,9% ,5.4% ）of Peripla-neta americana could reduce generation of TGF-β1 in supernatant for 24 h （ P 〈 0.05 ）. As for 48 h, only high concentration serum con-taining extracts Ⅰ （ 15% ） deceased the content of TGF-β1 in supernatant. For 24 and 48 h,serum containing extracts Ⅱ couldn＇t re-duce the content of TGF-β1 in supernatant（P 〈 0.05 ）. Conclusion： It has definite effect on anti-hepatic fibrosis with serum containing extracts of Periplaneta americana in vitro. The mechanism may be related to inhibiting HSC propagation and reducing the production of TGF-β1.