柠檬百里香叶挥发油成分分析及对肝癌细胞毒性作用

Chemical Composition of Essential Oil from Thymus citriodorus and Its Toxic Effect on Liver Cancer Cells

目的:分析柠檬百里香叶挥发油的主要化学成分并研究其对肝癌细胞的毒性作用。方法:采用水蒸气蒸馏法从柠檬百里香叶中提取挥发油,利用GC-MS对其化学成分进行分析。共鉴定出其中32个组分,占总出峰面积的95.44%,主要成分有冰片(28.82%)、百里香酚(14.43%)、3,7-二甲基-1,6-辛二烯-3-醇(8.26%)、1-甲基-4-(α-羟基-异丙基)环己烯(8.23%)、樟脑萜(5.1%)等。MTT法检测其对肝癌HepG2细胞的毒性作用,激光共聚焦检测对NF-κβ65表达的影响。结果:挥发油抑制肝癌HepG2细胞生长的的IC50值为0.34%浓度;NF-κβ65表达的平均荧光强度为:对照组为323.25,2-10浓度精油组为84.18,2-11浓度挥发油组为197.93,2-12浓度挥发油组261.43。结论:柠檬百里香叶挥发油对HepG2细胞有很强毒性作用,其诱导细胞凋亡机制可能与NF-κβ65表达有关。

Objective： To analyze the chemical composition of essential oil from Thymus citriodorus and its toxic effect on liver cancer cells. Methods ： The essential oil from Thymus citriodorus leaves was extraeted by steam distillation, and GC-MS was used for an-alyzing chemical composition. 35 components were identified which accounted for 95.44% of the total peak area. The main compo-nents were horneol （28.82%）, thymol （ 14.43% ）, 3,7-dimethyl-1,6-octadiene-3-ol （8.26%）, 1-methyl-4-[α-hydroxy-isopropyl cyclohexene （ 8.23% ） and terpenes camphor （5.1% ） , et. al. The toxic effects on HepG2 cells and expression of NF-κβ65 were meas-ured by MTT assay and couiocal laser scanning microscopy, respectively. Results： The IC50 of HepG2 cells inhibition was 0.34 %. The mean fluorescence intensity of NF-κβ65 expression was as follows： control group 323.25, 2 ^-10 concentration group 84. 18, 2^-11 concentration group 197.93 and 2 ^-12 oncentration group 261.43. Conclusion： The essential oil from Thymus eitriodorus leaves has strong toxic effects. The induced apoptosis mechanism may be associated with the expression of NF-κβ65.