乌榄叶水提取物对大鼠离体心脏灌流的作用探究

Experimental study on the effect of the liquid extracts of canarium pimela leeth on the rats ’ ex vivo cardiac perfusion

目的探究乌榄叶水提取物对大鼠离体心脏的冠脉流量、心肌收缩力及心率的影响。方法采用Langendorff离体心脏灌流法,通过MedLab生物信号采集处理系统,观察药物对正常大鼠离体心脏的冠脉流量、心肌收缩力及心率的作用。结果乌榄叶水提取物对大鼠冠脉流量作用效果与丹参的类似,与生理盐水空白组比较差异有统计学意义,具有稳定和增加冠脉流量作用,给药后前6 min表现为加强心肌收缩力作用,而后均逐渐减弱恢复。心率在整个过程中持续下降,且下降速率快。结论乌榄叶水提取物对冠脉流量和心肌收缩力具有先增加后减少至给药前状态的双向作用,并有较快、较强的慢心率作用。结果提示乌榄叶可能有利于降低心脏耗氧量,维持心功能,起保护心肌的作用。

Objective To approach the effect of the liquid extracts of Canarium Pimela Leeth on the rats’ex vivo cardiac perfusion. Methods Coronary artery flow, cardiac contractility, HR, were recorded by MedLab-E system via Langendorff method. Results The numerical value about coronary artery flow cardiac contractility were rised first and then descented, which both higher than before drug-delivery. HR was descented fast in the whole process. Conclusion The extracts of Canarium Pimela Leeth had bidirectional effect that coronary artery flow cardiac contractility was first rised and then descented to the state before drug-delivery. And with the fast descending HR.it help to lowers oxygen-consumption, maintain heart function and protect the myocardium.