苗药验方皮部熏洗对骨关节炎大鼠软骨细胞凋亡与增殖的影响

Miao Medicine Prescription Skin Fumigation Effect on Apoptosis and Proliferation of Bone Arthritis in Rats

[目的]探讨苗药皮部熏洗法治疗骨关节炎(Osteoarthritis,OA)的部分机理。[方法]取42只健康清洁级大鼠,随机选取8只不造模作为正常对照组,其余34只采用4%木瓜蛋白酶关节腔注射建立大鼠OA模型,一周后随机选取2只检测模型成功与否,将造模成功的32只大鼠随机分为模型组、熏组、洗组和熏洗组,治疗4周后处死动物,切取关节,采用HE染色进行关节软骨病理评分(Mankin's评分),免疫组化染色法测定PCNA及TGF-β1的表达。[结果]Mankin's评分结果显示:与模型组比较,各治疗组均降低Mankin's评分(P<0.05,P<0.01),且熏洗组与熏组、洗组相比差异显著(P<0.01)。正常组有少量PCNA和TGF-β1阳性细胞的表达,模型组中表达明显,治疗组均比模型组升高(P<0.05),其中熏洗组与模型组比差异显著(P<0.01)。[结论](1)通过苗药皮部熏洗的干预,对OA模型大鼠的痛行为及膝关节活动有明显的改善作用。(2)苗药皮部熏洗通过上调软骨PCNA和TGF-β1的表达,促进软骨细胞的增殖,加速软骨的修复,达到防治OA的目的。(3)熏洗法治疗大鼠OA比单独运用熏法、洗法效果更明显。

[Objective] Investigate Miao medicine fumigation and washing treating skin osteoarthriti（Osteoarthritis,OA） for the partial mechanism.[Methods] As for the 42 healthy rats,the randomly selected 8 of them were not built into a model group but as a normal control group,and the remaining 34 were adopted to establish the rat model,regarded as OA model of 4% Papuan inter-articulation injection.After checking whether the randomly selected 2 de tection models were successful or not a week later,we adopted 32 model rats and randomly divided them in the model group,smoking group,washing and fumigation group and then after 4 weeks of treatment,we killed them and cut off their joints,using the HE staining for Mankin’s testing,the Im munohistochemistry staining method of PCNA and TGF-β1 in the determination of staining method.[Results] The Mankin’s testing results showd： com pared with the model group,the fumigation and washing group had significant difference.The normal group expressed a small amount of PCNA and the expression of TGF-β1 positive cells in the model group,the treatment group were higher than the model group（P0.05）,wherein the fumigation group compared with model group had significant difference（P0.01）.[Conclusion]（1） The intervention by Miao medicine fumigation on skin,knee Pain activi ty behavior of OA model rats had obvious improvement.（2） Miao medicine fumigation regulating cartilage skin PCNA and TGF-β1,promoted the proliferation of chondrocyte,accelerated the cartilage repair,and achievd the purpose of preventing OA.（3） The treatment of fumigation and washing group were more obvious than the single fumigation group and washing group.