八角茴香中莽草酸的提取纯化工艺

Study on the Extraction and Purification Craft of Shikimic Acid from Illicium verum Hook. f.

对四川、广西、海南、浙江4地的八角属植物中莽草酸的含量进行了测定,得到广西产八角茴香(Illicium verum Hook.f.)中莽草酸含量最高,为12.61%。对莽草酸的两种提取工艺进行了比较,得到水提取工艺效果较好,有利于莽草酸的后续纯化,最佳工艺为按水料质量比10∶1加水回流提取1.5 h,提取3次。莽草酸的纯化工艺有萃取法、阳离子树脂交换法、阴离子树脂交换法、硅胶吸附法等,其各有利弊,综合分析以树脂交换法为佳。

The shikimic acid content in Illicium plants from Sichuan,Guangxi,Hainan,Zhejiang were measured.The results showed that star anise（Illicium verum Hook.f.） from Guangxi had the highest shikimic acid content,which was 12.61%.Two extraction process for shikimic acid were compared,and it was concluded that water extraction method had better extraction efficiency and was benefit for later purification of shikimic acid.The optimum craft was,mass ratio of material to water at 10∶1 and reflux extracting for 1.5 h.Methods for shikimic acid purification included extraction method,cation exchange resin method,anion exchange resin method,silica gel adsorption method and so on.Each method had advantages and disadvantages.According to comprehensive analysis,resin exchange method was better.