棉花病害拮抗菌SC-4鉴定及其抗菌物质分离纯化

Identification of a strain SC-4antagonizing cotton disease and analysis of the antifungal substance

从徐州工程学院周围多点取土样,筛选得到一株对多种细菌、真菌(尤其是引起棉花病害的真菌,如棉花立枯病菌、棉花枯萎病菌、棉铃红腐病菌)具有较好抑制作用的放线菌菌株,命名为SC-4.通过形态学、生理生化实验以及菌株16SrDNA序列分析,初步确定其为淡紫灰链霉菌类群中的灰褐链霉菌.通过硫酸铵沉淀获得样品,再经高效液相色谱分离,获得4个组分,其中组分2对棉花致病菌有较好的抑制作用.该研究对于新型生物农药的开发以及棉花病害生物防治具有一定意义.

Getting soil samples from different spots around Xuzhou Institute of Technology, a strain with high-yield- ing antimicrobial substances was screened. In particular, it can well control the growth of cotton pathogens, such as Fusarium oxysporum f. sp. vasinfectum and Fusariurn moniliforrne. This strain was named SC-4 and later identified as Streptomyces lavendulae by morphological, physiological and biochemical properties, 16S rDNA gene sequence analysis. The sample obtained from ammonium sulfate precipitation, was separated through HPLC. Four components was obtained and component 2 can antagonize cotton disease. This study is meaningful for new biology pesticide developing and cotton disease biocontrol.