摘要
建立快速检测违法添加剂奥沙普嗪的免疫学方法。以活泼酯法将奥沙普嗪分别连接到BSA和OVA上制备人工抗原,免疫家兔制备多克隆抗体,经条件优化建立竞争抑制酶联免疫吸附检测方法。结果显示:检测体系IC50值为3.1 ng/mL,最低检测限为0.5 ng/mL,与8种相关药物交叉反应率都小于0.1%。用ELISA方法与HPLC检测30种市售保健酒,1例阳性和29例阴性结果全部吻合。
To develop an immunoassay for detection of oxaprozin, oxaprozin was conjugated to BSA and OVA to synthesize artificial antigens using active ester method. Polyclonal antibodies were obtained from immunized rabbits. The IC50 value and LOD of the optimal ELISA were 3.1 ng/mL and 0.5 ng/mL, respectively. All cross reactivities against 8 associate drugs were less than 0.1%. The ELISA was in good agreement with LC-UV when detected oxaprozin in 1 positive and 29 negative health liquors from market.
出处
《食品研究与开发》
CAS
北大核心
2013年第11期48-50,86,共4页
Food Research and Development
基金
香港铭源基金科研项目(2010/188)
广东省营养膳食与健康重点实验室开放基金项目(2011K004)
广东省自然科学基金项目(S2011040001362)
关键词
功能食品
奥沙普嗪
违法添加
人工抗原
免疫检测
functional foods
oxaprozin
lllegal adulterant
artificial antigen
immunoassay
