人乳头瘤病毒感染和ras基因突变与喉鳞癌的关系

HUMAN PAPILLOMAVIRUS AND RAS GENE MUTATION IN LARYNGEAL SQUAMOUS CELL CARCINOMA

1目的 探讨人乳头瘤病毒 16 ,18型 (HPV16 ,18)感染及 ras基因突变与喉鳞癌的关系。2方法 应用聚合酶链反应 (PCR)和限制性片段长度多态性 (RFL P)分析技术 ,检测 40例喉鳞状细胞癌组织中 ras基因 (N-ras,K- ras,H- ras)第 12密码子的突变情况及 HPV16 ,18的感染情况。3结果 40例标本中检出 HPV16阳性 14例(35 % ) ,HPV18阳性 4例 (10 % )。 9例标本 ras基因发生突变 ,其中 N- ras6例 ,K- ras2例 ,H- ras1例。ras基因在HPV16阳性和阴性标本中的突变率分别为 11.11% (2 / 18)和 31.82 % (7/ 2 2 ) ,二者差异无显著意义 (χ2 =1.3917,P>0 .0 5 )。 4结论 HPV感染和 ras基因突变在喉鳞癌的发生发展过程中起重要作用。

Objective To evaluate the role of human papillomavirus type 16,18(HPV 16,18) infection and ras activation in laryngeal squamous cell carcinoma and to investigate the association between HPV and ras gene mutations. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism(RFLP) analysis techniques were used to detect codon 12 mutation in ras gene (N ras, K ras, H ras) and HPV16,18 infection in 40 largngeal squamous cell carcinoma specimen. Results The positive infection rate of HPV16 and 18 were 35%(14 cases) and 10%(4 cases) respectively. 9 of 40 cases(22.5%) revealed ras mutations(6 in N ras, 2 in K ras and 1 in H ras). The rate of ras gene mutations in HPV positive patients and HPV negative patients were 11.11%(2/18) and 31.82%(7/22) respectively. There was no significant difference between them(χ 2= 1.391 7 ,P>0.05). Conclusion HPV infection and ras activation are two important factors in laryngeal carcinogensis, but there is no correlation between HPV infection and ras gene mutations. [