阿昔洛韦血浆浓度的自动柱切换高效液相色谱法测定

Determination of acyclovir in human plasma by automated column-switching HPLC

目的 :建立适应大批量样品快速自动分析的阿昔洛韦血药浓度HPLC测定方法。方法 :应用自制自动磁力定时切换装置。分析柱采用WatersNova PakC18柱 (15 0mm× 3.9mm ,4μm) ,分析流动相为甲醇 - 0 .0 1mol·L-1磷酸氢二钾缓冲液 =3∶97(v/v) ,流速 1ml·min-1;预处理柱为 μBondapakC18柱 (5 0mm× 4.6mm ,37～ 5 0 μm) ,预处理流动相 (PMP)为含0 .0 1mol·L-1庚烷磺酸钠的磷酸缓冲液 ,流速 1ml·min-1;检测波长 2 5 4nm。结果 :阿昔洛韦血药浓度在 40～ 12 80ng·ml-1线性良好(r =0 .9998) ,最低检测限约为 2ng(S/N≥ 3) ,最低血浆药物检测浓度为 8ng·ml-1;绝对和相对回收率分别为 (91.2± 8.3) %和 (98.3± 5 .5 ) % ;日内及日间精密度都小于 10 %。结论 :方法简便、快速、灵敏、准确 ,适于大批量血样的分析。

OBJECTIVE:To establish an automated HPLC method for determination of acyclovir in human plasma.METHODS:A self-made automated column-switching control system was used.The analytical column (150 mm× 3.9 mm) was packed with μ Waters Nova-pak C 18 (4 μm) and the pre-column (50 mm× 4.6 mm) was packed with μ Bondapak C 18 (37～50 μm).Methanol-0.01 mol·L -1 dipotassium hydrogen phosphate (3∶97,v/v) was used as analytical mobile-phase and a phosphate buffer with 0.01 mol·L -1 sodium heptane sulfonate was used as pre-mobile-phase.The determination wavelength was 254 nm.RESULTS:The concentration of acyclovir in human plasma was linear with the area of chromatographic peak in 40～1280 ng·ml -1 ,the determination limit was 10 ng·ml -1 .The absolute and relative recoveries were ( 91.2 ± 8.3 )% and ( 98.3 ± 5.5 )%,respectively.The RSD were less than 10%.CONCLUSIONS:The method is simple,rapid,sensitive and accurate,which can apply to analysis of plenty of plasma samples.