摘要
目的研究 Bcl-2家族蛋白在乙醇引发肝细胞凋亡时的作用。方法以 TUNEL法、 DNA梯度检测乙醇诱导HepG_2肝细胞凋亡,以免疫组织化学法检测细胞中Bcl-2、Bax、Bak等蛋白表达情况,并以Bcl-2腺病毒感染细胞观察其抗凋亡作用。结果体积分数 0.2%、 1.0%乙醇处理 24 h对 HePG_2细胞无明显细胞毒性作用;3.0%组则有明显的细胞毒性作用,伴有典型的凋亡特征。免疫组织化学发现Bax、Bak蛋白在3.0%组显著增加;Bcl-2腺病毒载体可部分抑制凋亡。结论 Bax、Bak蛋白加强表达在乙醇诱导HeoG_2细胞凋亡中起一定作用; Bcl-2腺病毒载体可有效抑制乙醇诱导的凋亡。
Objective To evaluate the role of Bcl-2 family proteins in hepatocytic apoptosis caused by ethanol. Methods We induced hepatocytic apoptosis in HepG_2 cells with ethanol and detected the apoptosis with TUNEL assay. Bcl-2, Bax, and Bak protein expression in the hepatocytes was determined by immunohistochemistry method. We also observed the expression of Bcl-2 protein in HepG_2 cells infected with Bcl-2 adenovirus vector and its protection against hepatocytic apoptosis caused by ethanol. Results No obvious cytotoxicity was noted in 0.2% and l% ethanol treatment group, while it developed in 3% ethanol treatment group and accompanied by a marked expression of Bax, Bak proteins. Conclusion The overexpression of Bax, Bak proteins may play a role in HepG_2 cell apoptosis induced by ethanol and can be blocked effectively by Bcl-2 adenovirus vector.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第4期215-217,共3页
Chinese Journal of Hepatology
基金
国家自然科学基金!(367634)
卫生部基金