p21、CD44v在肺癌组织中的表达及其意义

Expressions of p21 and CD44v in human lung cancer and their significance

目的 探讨p2 1、CD44v蛋白在肺癌中可能的临床意义及相互间的关系。方法 采用流式细胞术 (FCM )检测 2 9例冻存的肺癌及 10例相应远离肿瘤部位的正常肺组织中p2 1、CD44v蛋白的表达。结果 肺癌组织中p2 1、CD44v的阳性率分别为 75 .9%和 5 1.7% ,两者相对含量 (FI值 )和阳性率均明显高于远癌肺组织 (P均 <0 .0 1)。p2 1和CD44v蛋白的表达明显相关 (P <0 .0 1)。p2 1的表达与肺癌分型、原发肿瘤大小、淋巴结转移、分期、分化程度等临床指标之间均无明显关系。CD44v表达在肺癌Ⅱ +Ⅲ期 ( 83 .3 % )高于Ⅰ期 ( 2 9.4% ) (P <0 .0 1) ,在伴有淋巴结转移的原发性肺癌 ( 81.8% )高于不伴有转移的肺癌 ( 3 3 .3 % ) (P <0 .0 5 )。结论 p2 1、CD44v异常表达在肺癌中可能具有一定的协同作用。CD44v阳性可能预示肺癌发生淋巴结转移的倾向 ,具有一定的临床意义。

Objective To investigate the clinical role of p21 and CD44v in primary lung cancer and their relationship with each other. Methods p21 and CD44v expression in 29 frozen lung cancer and 10 corresponding non cancer tissues were measured by flow cytometry (FCM). Results In lung cancer, the expression rates of p21 and CD44v were 75.9% and 51.7% respectively. The two proteins showed much higher FI values and positive rates in lung cancer than those in non cancer tissues (P<0.01). p21 alteration was independent from tumor size,lymph node metastasis, TNM stage, differentiated grade and histological type. CD44v expression in stage Ⅱ+Ⅲ(83.3%) was much higher than that in stage Ⅰ(29.4%) (P<0.01). As compared with lung cancer without lymph node metastasis (33.3%), primary lung cancer with lymph node metastasis showed significantly higher expression rate of CD44v (81.8%) (P<0.05). Coexpression of p21 and CD44v had a distinct statistic significance (P<0.01). Conclusion Abnormality of p21 and CD44v may be involved in the carcinogenesis and development of lung neoplasms in coordination. Immunopositivity of CD44v may suggest a potential of high risk for lymph node metastasis in lung cancer.