婴幼儿咽喉乳头瘤组织人乳头瘤病毒感染的探讨

The study of human papilloma viruses infection in juvenile pharynlaryngeal papillomatosis by PCR and dot blot hybridization

目的 探讨温州地区人乳头瘤病毒感染和婴幼儿咽喉乳头状瘤的关系。方法 应用聚合酶链反应和核酸斑点杂交技术检测 35例婴幼儿咽喉乳头瘤组织和 10例对照组组织 (小儿声带小结 )HPV6、11、16、18、3 3 5个型别的DNA。结果 乳头瘤组织HPV感染率为 91 4% (30 / 35 ) ,其中HPV6型检出率为 5 4 2 % (19/ 35 ) ,HPV11型感染率为 2 5 7% (9/ 35 ) ,多重型别HPV6+ 11感染率为 11 4% (4/ 35 ) ;HPV16、18、3 3 型 ,均为阴性。对照组各型检测结果均为阴性 ,两者对比差异有高度显著性 (P <0 0 0 1)。结论 温州地区婴幼儿咽喉乳头瘤的发生与HPV感染密切相关 ,尤以HPV6感染为主。PCR结合核酸斑点杂交技术检测HPV具有敏感性高、特异性强的优点 ,值得推广。

Objective To study the relationship between infection of human papillomavirus and juvenile pharynlaryngeal papilloma Methods Polymerase chain reaction (PCR) and dot blot hybridization were used to detect HPV 6,11,16,18,33 DNA of 35 samples of pharynlaryngeal papilloma and 10 samples of vocal nodule Results The positive rate of HPV in pharynlaryngeal papilloma was 91 4%(30/35) Among them the positive rates of HPV 6 and HPV 11 were 54 2%(19/35) and 25 7%(9/35), the positive rate of multiple types of HPV 6+11 was 11 4%(4/35) The positive rate of HPV 16,18,33 was negative The positive rate of HPV in vocal nodule was negative and were significantly different from that of the pharynlaryngeal papilloma group Conclusion The results suggest that infection with HPV, especially with HPV 6, is closely associated with the development of juvenile pharynlaryngeal papilloma in Wenzhou area HPV-DNA detected by PCR and dot blot hybridization has highly specificity and sensitivity