女贞子等中药对小鼠毛囊生长周期中生长因子mRNA表达的影响

Expression of Growth Factor mRNA in Hair Follicles Cultured with Some Chinese Herbs

目的 探讨女贞子等中药促毛囊生长的机制。方法 我们应用 RT-PCR技术和凝胶密度扫描分析技术，对正常生长周期中毛囊肝细胞生长因子 (HGF)和血管内皮细胞生长因子 (VEGF)mRNA表达水平，以及女贞子等中药对体外培养小鼠触须毛囊 HGF和 VEGF mRNA表达水平的影响进行了深入的研究。结果 我们的研究发现正常毛囊生长周期中，生长期毛囊有明显的 HGF和 VEGF mRNA表达，而退行 /休止期毛囊则很难检测出上述因子 mRNA的表达。在毛囊生长发育的过程中， HGF和 VEGF mRNA表达也逐渐减弱。与女贞子混合培养的毛囊，在培养的第 6天仍有相当量的 HGF和 VEGF mRNA表达，而相应的对照组则未能测到这些因子的表达。而与齐敦果酸培养的毛囊在培养的第 6天仅有 HGF mRNA表达，而无 VEGF mRNA的表达。结论 女贞子及齐敦果酸可促进体外培养的毛囊对 HGF和 (或 )VEGF mRNA的表达。从而从分子生物学水平揭示了上述药物促进毛囊生长的可能机制。

Objective To investigate the molecular mechanism of hair growth stimulated by some Chinese herbs. Methods Expression of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) mRNA was detected by RT-PCR in normal hair cycles and in hair follicles cultured with some Chinese herbs. Results Expression of HGF and VEGF mRNA varied in normal mouse vibrissa follicles during hair growth cycles. HGF and VEGF mRNA were detectable in anagen follicles, but not in catagen/telogen follicles. The expression of HGF and VEGF mRNA decreased gradually in the follicles from very early anagen to late anagen. At the sixth day the expression of HGF and VEGF mRNA was still detectable for the group in which the follicles were cultured with Ligustrum lucidum(A), but not for control group. At the sixth day HGF mRNA expressed only when the follicles cultured with olianolic acid, an active ingredient of Ligustrum lucidum. Conclusion The results demonstrate that there is varying expression of HGF and VEGF mRNA in the hair growth cycles of mouse vibrissa follicles. Ligustrum lucidum (A) is shown to up regulate the expression of HGF and VEGF in mouse vibrissa follicles culture model.