裸鼠重建毛囊的组织学研究

Histological Study of Reconstructed Hair Follicles

目的 观察毛乳头细胞诱导毛囊再生情况和毛囊重建情况。方法 采用器官型培养技术和裸鼠移植技术，将培养的毛囊毛乳头细胞、真皮鞘细胞和头皮 (或包皮 )真皮成纤维细胞分别制成胶原凝胶，再接种于毛囊上皮上段、下段和球部细胞，进行体外培养和裸鼠移植试验。结果 在毛囊毛乳头细胞与毛囊各段上皮细胞的器官型培养中均可见毛囊样结构形成；毛囊真皮鞘细胞凝胶上培养的毛球部细胞也重建出毛囊样结构。移植到裸鼠皮下后则可见较为完整的毛囊结构形成。结论 低传代培养的毛乳头细胞在体内外均具有诱导毛囊再生的能力。

Objective To observe the ability of dermal papilla cells inducing hair follicle regeneration and hair follicle reconstruction. Methods Gels of typeⅠ collagen(rat tail) separately populated with hair papillary cells(DPC), dermal sheath cells(DSC) or dermal fibroblasts were prepared. After contraction gels were mounted in a small tube. The epithelial cells of hair follicle were seeded onto these gels, grew in DMEM medium for a week, and lifted at the air medium interface and grew for an additional week, then grafted into the subcutis of nude mice. Results The formation of hair follicle like structure was observed in the combined culture of DPC with the cells of the upper fragment, lower portion and bulb of follicles or in organ culture of DSC with bulb cells. Nevertheless, when the cultures were implanted into the subcutis of nude mice, the relatively intact hair follicles were formed. Conclusion DPC can induce hair follicle regeneration and sustain hair follicle growth in vivo and in vitro.