氯丙嗪在紫外线诱导下致溶血反应的实验研究

Study on Chlorpromazine Induced Photohemolysis Under Exposure to UV-irradiation in Vitro

目的 监测因服药物而引起的皮肤光敏损害。方法 建立了以红细胞为底物，长波紫外线（ UVA）、中波紫外线（ UVB）为光源，检测光敏药物氯丙嗪光毒性的光溶血反应方法。 结果 研究发现氯丙嗪在 UVA和 UVB的照射下，随着照射剂量和药物浓度的增加，溶血现象越来越严重，但当照射剂量到一定数值时溶血现象达一恒定值。而氯丙嗪未经 UVA和 UVB照射则不造成溶血反应。结论 该方法的建立对于预检市场上不断增多的药物中有无光毒性物质，监测和控制药物性光毒性皮肤病的发生提供实验依据。

Objective To develop an in vitro system to monitor drug induced cutaneous photosensitive damage. Methods Photohemolysis test was used to assess the phototoxicity of chlorpromazine. Results It was shown that hemolysis was induced by chlorpromazine under exposure to irradiation of UVA and UVB. The intensity of hemolysis increased along with the rise of UVA and UVB dosage and chlorpromazine concentration. Hemolysis could not be induced without exposure to UVA and UVB irradiation. Conclusion It is suggested that this test system could be used to detect and monitor drugs with potential phototoxicity.